ArticlePloS one2010
Light sheet microscopy for single molecule tracking in living tissue.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
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Who cites it
66 citing papers in PubMed.
- Review of Machine Learning for Single-Particle Tracking: Methods, Challenges, and Biophysical Insights.Chemical & biomedical imaging · 2026Review
- Single-Molecule Localization Microscopy in vivo at the Drosophila Neuromuscular Junction.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
- Viewing early life without labels: optical approaches for imaging the early embryo†.Biology of reproduction · 2024Review
- Multimodal illumination platform for 3D single-molecule super-resolution imaging throughout mammalian cells.Biomedical optics express · 2024Article
- Interferometric modulation for generating extended light sheet: improving field of view.Journal of biomedical optics · 2024Article
- Real-time study of spatio-temporal dynamics (4D) of physiological activities in alive biological specimens with different FOVs and resolutions simultaneously.Scientific reports · 2024Article
- Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging.Journal of imaging · 2023Review
- Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Shining the Light on Astrocytic Ensembles.Cells · 2023Review
- Single-molecule tracking of myelin basic protein during oligodendrocyte differentiation.Biological imaging · 2023Article
- Characterizing Properties of Biomolecular Condensates Below the Diffraction Limit In Vivo.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Phase Separation-Mediated Chromatin Organization and Dynamics: From Imaging-Based Quantitative Characterizations to Functional Implications.International journal of molecular sciences · 2022Review
- Following the tracks: How transcription factor binding dynamics control transcription.Biophysical journal · 2022Review
- Lasers in Live Cell Microscopy.International journal of molecular sciences · 2022Review
- Photonics tools begin to clarify astrocyte calcium transients.Neurophotonics · 2022Review
- Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy.Biomedical optics express · 2021Article
- Light Sheet Illumination for 3D Single-Molecule Super-Resolution Imaging of Neuronal Synapses.Frontiers in synaptic neuroscience · 2021Review
- Fast multi-directional DSLM for confocal detection without striping artifacts.Biomedical optics express · 2020Article
- Single-Virus Tracking: From Imaging Methodologies to Virological Applications.Chemical reviews · 2020Review
6 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single molecule observation in cells and tissue allows the analysis of physiological processes with molecular detail, but it still represents a major methodological challenge. Here we introduce a microscopic technique that combines light sheet optical sectioning microscopy and ultra sensitive high-speed imaging. By this approach it is possible to observe single fluorescent biomolecules in solution, living cells and even tissue with an unprecedented speed and signal-to-noise ratio deep within the sample. Thereby we could directly observe and track small and large tracer molecules in aqueous solution. Furthermore, we demonstrated the feasibility to visualize the dynamics of single tracer molecules and native messenger ribonucleoprotein particles (mRNPs) in salivary gland cell nuclei of Chironomus tentans larvae up to 200 microm within the specimen with an excellent signal quality. Thus single molecule light sheet based fluorescence microscopy allows analyzing molecular diffusion and interactions in complex biological systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.