Evidence map›Paper›PMID 20668126›Full record

ArticleJournal of clinical microbiology2010

Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of clinically important yeast species.

Lindsay G Stevenson, Steven K Drake, Yvonne R Shea, Adrian M Zelazny, Patrick R Murray

Abstract readEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
102citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

102 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
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  5. Insights from Mass Spectrometry-Based Proteomics onJournal of fungi (Basel, Switzerland) · 2025
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42 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Lindsay G StevensonMicrobiology Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892-1508, USA.
Steven K Drake
Yvonne R Shea
Adrian M Zelazny
Patrick R Murray

Funding

Identification of Clinically Important Microbes by Genomic and Proteomic MethodsZIACL080012 · CLC · CLINICAL CENTER · PI ZELAZNY, ADRIAN MARCELO · 2009 to 2025
$0k
Molecular and Virulence Traits of Bacteria & Fungi Causing Persistent InfectionsZIACL080013 · CLC · CLINICAL CENTER · PI ZELAZNY, ADRIAN MARCELO · 2009 to 2025
$0k
Intramural NIH HHS
6 · The paper itself

Abstract

We evaluated the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the rapid identification of yeast species. Using Bruker Daltonics MALDI BioTyper software, we created a spectral database library with m/z ratios of 2,000 to 20,000 Da for 109 type and reference strains of yeast (44 species in 8 genera). The database was tested for accuracy by use of 194 clinical isolates (23 species in 6 genera). A total of 192 (99.0%) of the clinical isolates were identified accurately by MALDI-TOF MS. The MALDI-TOF MS-based method was found to be reproducible and accurate, with low consumable costs and minimal preparation time.

Indexed as

HumansMycologyMycosesReproducibility of ResultsSensitivity and SpecificitySpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTime FactorsYeasts

Identifiers

PMID20668126
PMCPMC2953094

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.