ArticlePlant physiology2010
Multiple regulatory elements in the Arabidopsis NIA1 promoter act synergistically to form a nitrate enhancer.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 57 citations in OpenAlex.
- Sentinel plants enable quantitative monitoring of bioavailable nitrate in soils and microbial environments.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Vacuum and Sonication Treatment Enable Efficient Transient Gene Expression in Various Monocot and Eudicot Plant Seedlings.ACS synthetic biology · 2026Article
- Progress and new questions in understanding the mechanisms controlling plant nitrogen responses.Plant biotechnology (Tokyo, Japan) · 2026Article
- Conservation and divergence of regulatory architecture in nitrate-responsive plant gene circuits.The Plant cell · 2025Article
- Exploring the genetic landscape of nitrogen uptake in durum wheat: genome-wide characterization and expression profiling of NPF and NRT2 gene families.Frontiers in plant science · 2023Article
- Coregulation ofFrontiers in plant science · 2023Article
- Article
- OsTBP2.1, a TATA-Binding Protein, Alters the Ratio ofInternational journal of molecular sciences · 2022Article
- Sulfur signaling and starvation response in Arabidopsis.iScience · 2022Review
- A Ratiometric Dual Color Luciferase Reporter for Fast Characterization of Transcriptional Regulatory Elements in Plants.ACS synthetic biology · 2021Article
- Article
- Interacting TCP and NLP transcription factors control plant responses to nitrate availability.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Dancing with Hormones: A Current Perspective of Nitrate Signaling and Regulation inFrontiers in plant science · 2017Review
- The Arabidopsis NRG2 Protein Mediates Nitrate Signaling and Interacts with and Regulates Key Nitrate Regulators.The Plant cell · 2016Article
- Synthetic promoters in planta.Planta · 2015Review
- The NAC transcription factor family in maritime pine (Pinus Pinaster): molecular regulation of two genes involved in stress responses.BMC plant biology · 2015Article
- Two short sequences in OsNAR2.1 promoter are necessary for fully activating the nitrate induced gene expression in rice roots.Scientific reports · 2015Article
- Nitrate foraging by Arabidopsis roots is mediated by the transcription factor TCP20 through the systemic signaling pathway.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Hypoosmotic expression of Dunaliella bardawil ζ-carotene desaturase is attributed to a hypoosmolarity-responsive element different from other key carotenogenic genes.Plant physiology · 2014Article
- Functional evolution of cis-regulatory modules of STMADS11 superclade MADS-box genes.Plant molecular biology · 2013Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
To accommodate fluctuating nutrient levels in the soil, plants modulate their metabolism and root development via signaling mechanisms that rapidly reprogram the plant transcriptome. In the case of nitrate, over 1,000 genes are induced or repressed within minutes of nitrate exposure. To identify cis-regulatory elements that mediate these responses, an enhancer screen was performed in transgenic Arabidopsis (Arabidopsis thaliana) plants. A 1.8-kb promoter fragment from the nitrate reductase gene NIA1 was identified that acts as a nitrate enhancer when fused to a 35S minimal promoter. Enhancer activity was localized to a 180-bp fragment, and this activity could be enhanced by the addition of a 131-bp fragment from the nitrite reductase promoter. A promoter construct containing the 180- and 131-bp fragments was also induced by nitrite and repressed by ammonium, indicating that it was responsive to multiple nitrogen signals. To identify specific regulatory elements within the 180-bp NIA1 fragment, a transient expression system using agroinfiltration of Nicotiana benthamiana was developed. Deletion analysis identified three elements corresponding to predicted binding motifs for homeodomain/E-box, Myb, and Alfin1 transcription factors. A fully active promoter showing nitrate and nitrite enhancer activity equivalent to that of the wild-type 180-bp fragment could be built from these three elements if the spacing between the homeodomain/E-box and Myb-Alfin1 sites was equivalent to that of the native promoter. These findings were validated in transgenic Arabidopsis plants and identify a cis-regulatory module containing three elements that comprise a nitrate enhancer in the NIA1 promoter.
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