ArticleVeterinary microbiology2011
Comparison of phenotypic and genotypic methods for the species identification of coagulase-negative staphylococcal isolates from bovine intramammary infections.
Article in Veterinary microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 66 citations in OpenAlex.
- Non-aureus staphylococci and mammaliicocci as a cause of mastitis in domestic ruminants: current knowledge, advances, biomedical applications, and future perspectives - a systematic review.Veterinary research communications · 2023Pooled it
- Genus-targeted markers for the taxonomic identification and monitoring of coagulase-positive and coagulase-negative Staphylococcus species.World journal of microbiology & biotechnology · 2024Article
- Characterization of Biofilm Producing Coagulase-Negative Staphylococci Isolated from Bulk Tank Milk.Veterinary sciences · 2022Article
- Virulence Factors inAntibiotics (Basel, Switzerland) · 2021Article
- Characterization of genetic diversity and population structure within Staphylococcus chromogenes by multilocus sequence typing.PloS one · 2021Article
- Recognition of (Sesc) for Easy Identification ofReports of biochemistry & molecular biology · 2020Article
- Amplicon-Based High-Throughput Sequencing Method Capable of Species-Level Identification of Coagulase-Negative Staphylococci in Diverse Communities.Microorganisms · 2020Article
- Efficacy of MALDI-TOF mass spectrometry as well as genotypic and phenotypic methods in identification of staphylococci other thanJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- Indoor Air Quality and Potential Health Risk Impacts of Exposure to Antibiotic Resistant Bacteria in an Office Rooms in Southern Poland.International journal of environmental research and public health · 2018Article
- Identification and characterization of Staphylococcus devriesei isolates from bovine intramammary infections in KwaZulu-Natal, South Africa.BMC veterinary research · 2018Article
- Article
- Improved multiplex PCR primers for rapid identification of coagulase-negative staphylococci.Archives of microbiology · 2018Article
- Species Identification and Strain Typing of Staphylococcus agnetis and Staphylococcus hyicus Isolates from Bovine Milk by Use of a Novel Multiplex PCR Assay and Pulsed-Field Gel Electrophoresis.Journal of clinical microbiology · 2017Article
- Local host response following an intramammary challenge with Staphylococcus fleurettii and different strains of Staphylococcus chromogenes in dairy heifers.Veterinary research · 2016Article
- Determining the Presence of Superantigens in Coagulase Negative Staphylococci from Humans.PloS one · 2015Article
- Dominant obligate anaerobes revealed in lower respiratory tract infection in horses by 16S rRNA gene sequencing.The Journal of veterinary medical science · 2014Article
- Investigations on the efficacy of routinely used phenotypic methods compared to genotypic approaches for the identification of staphylococcal species isolated from companion animals in Irish veterinary hospitals.Irish veterinary journal · 2013Article
- Article
- Characterization of fatty acid modifying enzyme activity in staphylococcal mastitis isolates and other bacteria.BMC research notes · 2012Article
- Molecular epidemiology of mastitis pathogens of dairy cattle and comparative relevance to humans.Journal of mammary gland biology and neoplasia · 2011Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Coagulase-negative staphylococci (CNS) are the most frequently isolated pathogens from cows with intramammary infection (IMI). Although API STAPH ID 20, a commercially available identification system, and PCR-restriction fragment length polymorphism (PCR-RFLP) of the gap gene (gap PCR-RFLP) have been successfully applied for the identification of CNS isolates from human specimens, their accuracy in the identification of veterinary isolates has not been fully established. In this study, we identified 263 CNS isolates from bovine IMI at species level by partial 16S rRNA gene sequence analysis as the definitive test. Species identification obtained using partial 16S rRNA gene sequence analysis was compared to results from the API STAPH ID 20 and gap PCR-RFLP analysis. Eleven different CNS species were identified by partial 16S rRNA gene sequence analysis. Only 76.0% (200/263) of the species identification results obtained by API STAPH ID 20 matched those obtained by partial 16S rRNA gene sequence analysis, whereas 97.0% (255/263) of the species identification results obtained by the gap PCR-RFLP analysis matched those obtained by partial 16S rRNA gene sequence analysis. The gap PCR-RFLP analysis could be a useful and reliable alternative method for the species identification of CNS isolates from bovine IMI and appears to be a more accurate method of species identification than the API STAPH ID 20 system.
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Registered trials
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