ArticleAnalytical biochemistry2010
A high-throughput differential filtration assay to screen and select detergents for membrane proteins.
Article in Analytical biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A 10-year meta-analysis of membrane protein structural biology: Detergents, membrane mimetics, and structure determination techniques.Biochimica et biophysica acta. Biomembranes · 2021Pooled it
- Biophysical Characterization of Membrane Proteins.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Review
- Changes in Membrane Protein Structural Biology.Biology · 2020Review
- Structure/Function Analysis of human ZnT8 (SLC30A8): A Diabetes Risk Factor and Zinc Transporter.Current research in structural biology · 2020Article
- High-throughput stability screening for detergent-solubilized membrane proteins.Scientific reports · 2019Article
- Article
- Interrogating Detergent Desolvation of Nanopore-Forming Proteins by Fluorescence Polarization Spectroscopy.Analytical chemistry · 2017Article
- Expression and purification of the alpha subunit of the epithelial sodium channel, ENaC.Protein expression and purification · 2016Article
- Large-scale identification of membrane proteins with properties favorable for crystallization.Protein science : a publication of the Protein Society · 2015Article
- Membrane proteins, detergents and crystals: what is the state of the art?Acta crystallographica. Section F, Structural biology communications · 2014Review
- Detergent optimized membrane protein reconstitution in liposomes for solid state NMR.Biochemistry · 2014Article
- Article
- The significance of G protein-coupled receptor crystallography for drug discovery.Pharmacological reviews · 2011Review
- Simple screening method for improving membrane protein thermostability.Methods (San Diego, Calif.) · 2011Article
- The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal.Protein expression and purification · 2011Article
- High throughput platforms for structural genomics of integral membrane proteins.Current opinion in structural biology · 2011Review
- Transmembrane signaling and assembly of the cytochrome b6f-lipidic charge transfer complex.Biochimica et biophysica actaReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Structural studies on integral membrane proteins are routinely performed on protein-detergent complexes (PDCs) consisting of purified protein solubilized in a particular detergent. Of all the membrane protein crystal structures solved to date, a subset of only four detergents has been used in more than half of these structures. Unfortunately, many membrane proteins are not well behaved in these four detergents and/or fail to yield well-diffracting crystals. Identification of detergents that maintain the solubility and stability of a membrane protein is a critical step and can be a lengthy and "protein-expensive" process. We have developed an assay that characterizes the stability and size of membrane proteins exchanged into a panel of 94 commercially available and chemically diverse detergents. This differential filtration assay (DFA), using a set of filtered microplates, requires sub-milligram quantities of purified protein and small quantities of detergents and other reagents and is performed in its entirety in several hours.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.