ArticleAIDS research and human retroviruses2010
Analysis of HIV diversity using a high-resolution melting assay.
Article in AIDS research and human retroviruses, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Association of HIV diversity and virologic outcomes in early antiretroviral treatment: HPTN 052.PloS one · 2017Trial
- Association of pol diversity with antiretroviral treatment outcomes among HIV-infected African children.PloS one · 2013Trial
- Rapid detection of the SARS-CoV-2 omicron variants based on high-resolution melting curve analysis.Scientific reports · 2024Article
- Rapid monitoring of SARS-CoV-2 variants of concern through high-resolution melt analysis.Scientific reports · 2023Article
- Development of a genotyping platform for SARS-CoV-2 variants using high-resolution melting analysis.Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy · 2021Article
- Mother-to-Child HIV Transmission Bottleneck Selects for Consensus Virus with Lower Gag-Protease-Driven Replication Capacity.Journal of virology · 2017Article
- Rapid Detection of Common HIV-1 Drug Resistance Mutations by Use of High-Resolution Melting Analysis and Unlabeled Probes.Journal of clinical microbiology · 2017Article
- Article
- Analysis of HIV Diversity in HIV-Infected Black Men Who Have Sex with Men (HPTN 061).PloS one · 2016Article
- Review
- How can we better identify early HIV infections?Current opinion in HIV and AIDS · 2015Review
- Development of a general method for detection and quantification of the P35S promoter based on assessment of existing methods.Scientific reports · 2014Article
- HIV diversity as a biomarker for HIV incidence estimation: including a high-resolution melting diversity assay in a multiassay algorithm.Journal of clinical microbiology · 2014Article
- Article
- Impact of mutation type and amplicon characteristics on genetic diversity measures generated using a high-resolution melting diversity assay.The Journal of molecular diagnostics : JMD · 2013Article
- Microbiological applications of high-resolution melting analysis.Journal of clinical microbiology · 2012Review
- Use of a high resolution melting assay to analyze HIV diversity in HIV-infected Ugandan children.The Pediatric infectious disease journal · 2012Article
- Comparison of a high-resolution melting assay to next-generation sequencing for analysis of HIV diversity.Journal of clinical microbiology · 2012Article
- Workshop summary: Novel biomarkers for HIV incidence assay development.AIDS research and human retroviruses · 2012Article
- Article
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Authors and funding
11 authors.
Funding
Abstract
HIV viruses are usually genetically homogeneous shortly after infection, and become more heterogeneous over time. We developed a high-resolution melting (HRM) assay to analyze HIV diversity without sequencing. Plasma samples from the HIVNET 012 trial were obtained from nine Ugandan mother-infant pairs. DNA amplified from the HIV gag region was analyzed to determine the number of degrees over which the DNA melted (HRM score). HRM gag DNA was also cloned and sequenced (50 clones/mother; 20 clones/infant). The median HRM score for infants (4.3, range 4.2-5.3) was higher than that for control plasmids (3.4, range 3.2-3.8, p < 0.001) and lower than that for mothers (5.7, range 4.4-7.7, p = 0.005, exact Wilcoxon rank sum test). The intraclass correlation coefficient reflecting assay reproducibility was 94% (95% CI: 89-98%). HRM scores were also compared to sequenced-based measures of HIV diversity; higher HRM scores were associated with higher genetic diversity (p < 0.001), complexity (p = 0.009), and Shannon entropy (p = 0.022), but not with length variation (p = 0.111). The HRM assay provides a novel, rapid method for assessing HIV diversity without sequencing. This assay could be applied to any region of the HIV genome or to other genetic systems that exhibit DNA diversity.
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