ArticleBiochemistry2010
Pre-steady state kinetic studies of the fidelity of nucleotide incorporation by yeast DNA polymerase delta.
Article in Biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Engineered polymerase-mediated efficient synthesis of site-specifically functionalized RNA and 2'-modified RNA oligonucleotides via genetic alphabet expansion.Nature communications · 2026Article
- Understanding the Fidelity and Specificity of DNA Polymerase I.ACS omega · 2025Article
- Article
- Structural and Molecular Kinetic Features of Activities of DNA Polymerases.International journal of molecular sciences · 2022Review
- Mechanistic cross-talk between DNA/RNA polymerase enzyme kinetics and nucleotide substrate availability in cells: Implications for polymerase inhibitor discovery.The Journal of biological chemistry · 2020Review
- Structural insights into mutagenicity of anticancer nucleoside analog cytarabine during replication by DNA polymerase η.Scientific reports · 2019Article
- Error-Speed Correlations in Biopolymer Synthesis.Physical review letters · 2019Article
- PCNA accelerates the nucleotide incorporation rate by DNA polymerase δ.Nucleic acids research · 2019Article
- Activity and fidelity of human DNA polymerase α depend on primer structure.The Journal of biological chemistry · 2018Article
- Plant organellar DNA polymerases are replicative and translesion DNA synthesis polymerases.Nucleic acids research · 2017Article
- Eukaryotic DNA Replication Fork.Annual review of biochemistry · 2017Review
- Resolving individual steps of Okazaki-fragment maturation at a millisecond timescale.Nature structural & molecular biology · 2016Article
- Pre-steady-state Kinetic Analysis of a Family D DNA Polymerase from Thermococcus sp. 9°N Reveals Mechanisms for Archaeal Genomic Replication and Maintenance.The Journal of biological chemistry · 2015Article
- Yeast DNA polymerase ϵ catalytic core and holoenzyme have comparable catalytic rates.The Journal of biological chemistry · 2015Article
- Significant contribution of the 3'→5' exonuclease activity to the high fidelity of nucleotide incorporation catalyzed by human DNA polymerase ϵ.Nucleic acids research · 2014Article
- Kinetic mechanism of DNA polymerization catalyzed by human DNA polymerase ε.Biochemistry · 2013Article
- Distinct structural alterations in proliferating cell nuclear antigen block DNA mismatch repair.Biochemistry · 2013Article
- PCNA trimer instability inhibits translesion synthesis by DNA polymerase η and by DNA polymerase δ.DNA repair · 2013Article
- Kinetic characterization of exonuclease-deficient Staphylococcus aureus PolC, a C-family replicative DNA polymerase.PloS one · 2013Article
- Thermodynamic basis for the emergence of genomes during prebiotic evolution.PLoS computational biology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Eukaryotic DNA polymerase delta (pol delta) is a member of the B family of polymerases and synthesizes most of the lagging strand during DNA replication. Yeast pol delta is a heterotrimer comprised of three subunits: the catalytic subunit (Pol3) and two accessory subunits (Pol31 and Pol32). Although pol delta is one of the major eukaryotic replicative polymerase, the mechanism by which it incorporates nucleotides is unknown. Here we report both steady state and pre-steady state kinetic studies of the fidelity of pol delta. We found that pol delta incorporates nucleotides with an error frequency of 10(-4) to 10(-5). Furthermore, we showed that for correct versus incorrect nucleotide incorporation, there are significant differences between both pre-steady state kinetic parameters (apparent K(d)(dNTP) and k(pol)). Somewhat surprisingly, we found that pol delta synthesizes DNA at a slow rate with a k(pol) of approximately 1 s(-1). We suggest that, unlike its prokaryotic counterparts, pol delta requires replication accessory factors like proliferating cell nuclear antigen to achieve rapid rates of nucleotide incorporation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.