ArticleChembiochem : a European journal of chemical biology2010
A single molecular beacon probe is sufficient for the analysis of multiple nucleic acid sequences.
Article in Chembiochem : a European journal of chemical biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 58 citations in OpenAlex.
- Hybridization-Based Electrochemical Biosensors for RNA Detection: Key Concepts and Insights.Sensors and actuators. B, Chemical · 2026Article
- DNA Nanomachine (DNM) Biplex Assay for DifferentiatingInternational journal of molecular sciences · 2023Article
- Toward a Home Test for COVID-19 Diagnosis: DNA Machine for Amplification-Free SARS-CoV-2 Detection in Clinical Samples.ChemMedChem · 2022Article
- Rapid, multiplexed, and nucleic acid amplification-free detection of SARS-CoV-2 RNA using an electrochemical biosensor.Biosensors & bioelectronics · 2022Article
- Cascade of deoxyribozymes for the colorimetric analysis of drug resistance in Mycobacterium tuberculosis.Biosensors & bioelectronics · 2020Article
- Toward a Rational Approach to Design Split G-Quadruplex Probes.ACS chemical biology · 2019Article
- Alphanumerical Visual Display Made of DNA Logic Gates for Drug Susceptibility Testing of Pathogens.Chembiochem : a European journal of chemical biology · 2018Article
- A mutation-resistant deoxyribozyme OR gate for highly selective detection of viral nucleic acids.Chemical communications (Cambridge, England) · 2017Article
- A Single Electrochemical Probe Used for Analysis of Multiple Nucleic Acid Sequences.Electroanalysis · 2017Article
- MicroRNA Detection Using a Double Molecular Beacon Approach: Distinguishing Between miRNA and Pre-miRNA.Theranostics · 2017Article
- Nonequilibrium Hybridization Enables Discrimination of a Point Mutation within 5-40 °C.Journal of the American Chemical Society · 2016Article
- Detection of bacterial 16S rRNA using a molecular beacon-based X sensor.Biosensors & bioelectronics · 2013Article
- Operating Cooperatively (OC) sensor for highly specific recognition of nucleic acids.PloS one · 2013Article
- Molecular logic gates for DNA analysis: detection of rifampin resistance in M. tuberculosis DNA.Angewandte Chemie (International ed. in English) · 2012Article
- Review
- DNA nanotechnology for nucleic acid analysis: DX motif-based sensor.Chembiochem : a European journal of chemical biology · 2011Article
- Molecular-beacon-based tricomponent probe for SNP analysis in folded nucleic acids.Chemistry (Weinheim an der Bergstrasse, Germany) · 2011Article
- Enzyme-assisted binary probe for sensitive detection of RNA and DNA.Chemical communications (Cambridge, England) · 2010Article
- Real-time SNP analysis in secondary-structure-folded nucleic acids.Angewandte Chemie (International ed. in English) · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Molecular beacon (MB) probes are dual-labeled hairpin-shaped oligodeoxyribonucleotides that are extensively used for real-time detection of specific RNA/DNA analytes. In the MB probe, the loop fragment is complementary to the analyte: therefore, a unique probe is required for the analysis of each new analyte sequence. The conjugation of an oligonucleotide with two dyes and subsequent purification procedures add to the cost of MB probes, thus reducing their application in multiplex formats. Here we demonstrate how one MB probe can be used for the analysis of an arbitrary nucleic acid. The approach takes advantage of two oligonucleotide adaptor strands, each of which contains a fragment complementary to the analyte and a fragment complementary to an MB probe. The presence of the analyte leads to association of MB probe and the two DNA strands in quadripartite complex. The MB probe fluorescently reports the formation of this complex. In this design, the MB does not bind the analyte directly; therefore, the MB sequence is independent of the analyte. In this study one universal MB probe was used to genotype three human polymorphic sites. This approach promises to reduce the cost of multiplex real-time assays and improve the accuracy of single-nucleotide polymorphism genotyping.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.