ArticleLab on a chip2010
Microdevices integrating affinity columns and capillary electrophoresis for multibiomarker analysis in human serum.
Article in Lab on a chip, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 62 citations in OpenAlex.
- Transformation gap from research findings to large-scale commercialized products in microfluidic field.Materials today. Bio · 2024Review
- Application of Silicon Nanowire Field Effect Transistor (SiNW-FET) Biosensor with High Sensitivity.Sensors (Basel, Switzerland) · 2023Review
- Air-Bubble-Insensitive Microfluidic Lactate Biosensor for Continuous Monitoring of Lactate in Sweat.ACS sensors · 2023Article
- Immunoaffinity monoliths for multiplexed extraction of preterm birth biomarkers from human blood serum in 3D printed microfluidic devices.The Analyst · 2022Article
- 3D printed microfluidic devices with immunoaffinity monoliths for extraction of preterm birth biomarkers.Analytical and bioanalytical chemistry · 2019Article
- High-Throughput Optofluidic Acquisition of Microdroplets in Microfluidic Systems.Micromachines · 2018Review
- Automated microfluidic devices integrating solid-phase extraction, fluorescent labeling, and microchip electrophoresis for preterm birth biomarker analysis.Analytical and bioanalytical chemistry · 2018Article
- Electrokinetically operated microfluidic devices for integrated immunoaffinity monolith extraction and electrophoretic separation of preterm birth biomarkers.The Analyst · 2017Article
- Integrated electrokinetically driven microfluidic devices with pH-mediated solid-phase extraction coupled to microchip electrophoresis for preterm birth biomarkers.Electrophoresis · 2017Article
- On-Chip Fluorescent Labeling using Reversed-phase Monoliths and Microchip Electrophoretic Separations of Selected Preterm Birth Biomarkers.Analytical methods : advancing methods and applications · 2016Article
- Current Technologies and Recent Developments for Screening of HPV-Associated Cervical and Oropharyngeal Cancers.Cancers · 2016Review
- Development of an integrated microfluidic solid-phase extraction and electrophoresis device.The Analyst · 2016Article
- A multiplexed immunoaggregation biomarker assay using a two-stage micro resistive pulse sensor.Biomicrofluidics · 2016Article
- Pressure-actuated microfluidic devices for electrophoretic separation of pre-term birth biomarkers.Analytical and bioanalytical chemistry · 2016Article
- Integrated, DC voltage-driven nucleic acid diagnostic platform for real sample analysis: Detection of oral cancer.Talanta · 2015Article
- On chip preconcentration and fluorescence labeling of model proteins by use of monolithic columns: device fabrication, optimization, and automation.Analytical and bioanalytical chemistry · 2015Article
- Microfluidic Valves Made From Polymerized Polyethylene Glycol Diacrylate.Sensors and actuators. B, Chemical · 2014Article
- Advances in microfluidic materials, functions, integration, and applications.Chemical reviews · 2013Review
- Microfluidic chips with reversed-phase monoliths for solid phase extraction and on-chip labeling.Journal of chromatography. A · 2012Article
- Single-monomer formulation of polymerized polyethylene glycol diacrylate as a nonadsorptive material for microfluidics.Analytical chemistry · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Biomarkers in human body fluids have great potential for use in screening for diseases such as cancer and diabetes, diagnosis, determining the effectiveness of treatments, and detecting recurrence. Present 96-well immunoassay technology effectively analyzes large numbers of samples; however, this approach is more expensive and less time effective on single or a few samples. In contrast, microfluidic systems are well suited for assaying small numbers of specimens in a point-of-care setting, provided suitable procedures are developed to work within peak capacity constraints when analyzing complex mixtures like human blood serum. Here, we developed integrated microdevices with an affinity column and capillary electrophoresis channels to isolate and quantitate a panel of proteins in complex matrices. To form an affinity column, a thin film of a reactive polymer was photopolymerized in a microchannel, and four antibodies were covalently immobilized to it. The retained protein amounts were consistent from chip to chip, demonstrating reproducibility. Furthermore, the signals from four fluorescently labeled proteins captured on-column were in the same range after rinsing, indicating the column has little bias toward any of the four antibodies or their antigens. These affinity columns have been integrated with capillary electrophoresis separation, enabling us to simultaneously quantify four protein biomarkers in human blood serum in the low ng mL(-1) range using either a calibration curve or standard addition. Our systems provide a fast, integrated and automated platform for multiple biomarker quantitation in complex media such as human blood serum.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.