ArticleMolecular vision2010
Gene expression profile of epithelial cells and mesenchymal cells derived from limbal explant culture.
Article in Molecular vision, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Regeneration and Cancer-The Two Sides of a "Stem Cell" Coin: The 2025 Mildred Weisenfeld Award Lecture.Investigative ophthalmology & visual science · 2026Article
- Immunophenotypical Characterization of Limbal Mesenchymal Stromal Cell Subsets during In Vitro Expansion.International journal of molecular sciences · 2024Article
- Electrospun poly(l-lactide-Heliyon · 2024Article
- Human Lacrimal Gland Derived Mesenchymal Stem Cells - Isolation, Propagation, and Characterization.Investigative ophthalmology & visual science · 2023Article
- Concise Review: Bioengineering of Limbal Stem Cell Niche.Bioengineering (Basel, Switzerland) · 2023Review
- Efficient Isolation and Functional Characterization of Niche Cells from Human Corneal Limbus.International journal of molecular sciences · 2022Article
- Bovine milk somatic cell transcriptomic response to Staphylococcus aureus is dependent on strain genotype.BMC genomics · 2021Article
- Current perspectives of limbal-derived stem cells and its application in ocular surface regeneration and limbal stem cell transplantation.Stem cells translational medicine · 2021Review
- Stem Cell Niche Microenvironment: Review.Bioengineering (Basel, Switzerland) · 2021Review
- Cutting corners, or simplifying technology to reach more patients; using the body as its own incubator for epithelial regeneration.Indian journal of ophthalmology · 2019Article
- Strategies for reconstructing the limbal stem cell niche.The ocular surface · 2019Review
- Limbal stem cells: identity, developmental origin, and therapeutic potential.Wiley interdisciplinary reviews. Developmental biology · 2018Review
- Limbal stromal cells derived from porcine tissue demonstrate mesenchymal characteristics in vitro.Scientific reports · 2017Article
- A Human Corneal Epithelial Cell Line Model for Limbal Stem Cell Biology and Limbal Immunobiology.Stem cells translational medicine · 2017Article
- Article
- Human limbal mesenchymal cells support the growth of human corneal epithelial stem/progenitor cells.Investigative ophthalmology & visual science · 2014Article
- Concise review: immunological properties of ocular surface and importance of limbal stem cells for transplantation.Stem cells translational medicine · 2013Review
- Hepatocyte growth factor mediates mesenchymal stem cell–induced recovery in multiple sclerosis models.Nature neuroscience · 2012Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeLimbal stem cell deficiency is a challenging clinical problem and the current treatment involves replenishing the depleted limbal stem cell (LSC) pool by either limbal tissue transplantation or use of cultivated limbal epithelial cells (LEC). Our experience of cultivating the LEC on denuded human amniotic membrane using a feeder cell free method, led to identification of mesenchymal cells of limbus (MC-L), which showed phenotypic resemblance to bone marrow derived mesenchymal stem cells (MSC-BM). To understand the transcriptional profile of these cells, microarray experiments were carried out.
methodsRNA was isolated from cultured LEC, MC-L and MSC-BM and microarray experiments were carried out by using Agilent chip (4x44 k). The microarray data was validated by using Realtime and semiquntitative reverse transcription polymerase chain reaction.
resultsThe microarray analysis revealed specific gene signature of LEC and MC-L, and also their complementary role related to cytokine and growth factor profile, thus supporting the nurturing roles of the MC-L. We have also observed similar and differential gene expression between MC-L and MSC-BM.
conclusionsThis study represents the first extensive gene expression analysis of limbal explant culture derived epithelial and mesenchymal cells and as such reveals new insight into the biology, ontogeny, and in vivo function of these cells.
Indexed as
Identifiers
20664697PMC2903463What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.