ArticleMolecular therapy : the journal of the American Society of Gene Therapy2010
Efficient gene transfer into the mouse lung by fetal intratracheal injection of rAAV2/6.2.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Tropism of adeno-associated virus serotypes in mouse lungs via intratracheal instillation.Virology journal · 2024Article
- Fetal hypoplastic lungs have multilineage inflammation that is reversed by amniotic fluid stem cell extracellular vesicle treatment.Science advances · 2024Article
- The technical and ethical framework of fetal therapy: past and current advances.Current stem cell reports · 2024Article
- A Minimally Invasive Method for Intratracheal Instillation of Drugs in Neonatal Rodents to Treat Lung Disease.Journal of visualized experiments : JoVE · 2021Article
- Delivery technologies for in utero gene therapy.Advanced drug delivery reviews · 2021Review
- Delivering on the promise of gene editing for cystic fibrosis.Genes & diseases · 2019Review
- Targeted Gene Delivery through the Respiratory System: Rationale for Intratracheal Gene Transfer.Journal of cardiovascular development and disease · 2019Review
- Gene Editing and Genetic Lung Disease. Basic Research Meets Therapeutic Application.American journal of respiratory cell and molecular biology · 2017Review
- A novel translational model for fetoscopic intratracheal delivery of nanoparticles in piglets.Prenatal diagnosis · 2016Article
- Immunological ignorance allows long-term gene expression after perinatal recombinant adeno-associated virus-mediated gene transfer to murine airways.Human gene therapy · 2014Article
- In utero lung gene transfer using adeno-associated viral and lentiviral vectors in mice.Human gene therapy methods · 2014Article
- Towards non-invasive monitoring of pathogen-host interactions during Candida albicans biofilm formation using in vivo bioluminescence.Cellular microbiology · 2014Article
- Recipient leukocyte infusion enhances the local and systemic graft-versus-neuroblastoma effect of allogeneic bone marrow transplantation in mice.Cancer immunology, immunotherapy : CII · 2013Article
- A novel surgical approach for intratracheal administration of bioactive agents in a fetal mouse model.Journal of visualized experiments : JoVE · 2012Article
- Jaagsiekte sheep retrovirus pseudotyped lentiviral vector-mediated gene transfer to fetal ovine lung.Gene therapy · 2012Article
- Bioluminescence imaging: progress and applications.Trends in biotechnology · 2011Review
- Genetic therapies for cystic fibrosis lung disease.Human molecular genetics · 2011Review
- Optical imaging in vivo with a focus on paediatric disease: technical progress, current preclinical and clinical applications and future perspectives.Pediatric radiology · 2011Review
- The fetal respiratory system as target for antenatal therapy.Facts, views & vision in ObGyn · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fetal gene therapy is one of the possible new therapeutic strategies for congenital or perinatal diseases with high mortality or morbidity. We developed a novel delivery strategy to inject directly into the fetal mouse trachea. Intratracheal (i.t.) injection at embryonic day 18 (E18) was more efficient in targeting the fetal lung than conventional intra-amniotic (i.a.) delivery. Viral vectors derived from adeno-associated virus serotype 6.2, with tropism for the airway epithelium and not earlier tested in the fetal mouse lung, were injected into the fetal trachea. Bioluminescence (BL) imaging (BLI) was combined with magnetic resonance (MR) imaging (MRI) for noninvasive and accurate localization of transgene expression in vivo. Histological analysis for β-galactosidase (β-gal) revealed 17.5% of epithelial cells transduced in the conducting airways and 1.5% in the alveolar cells. Stable gene expression was observed up to 1 month after injection. This study demonstrates that direct injection of rAAV2/6.2 in the fetal mouse trachea is superior to i.a. delivery for transducing the lung. Second, as stable gene transfer was detected up to 1 postnatal month, this approach may be useful to evaluate fetal gene therapy for pulmonary diseases such as cystic fibrosis, requiring both substantial numbers of transduced cells as well as prolonged gene expression to obtain a stable phenotypic effect.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.