ArticleThe Plant cell2010
The myosin motor domain of fungal chitin synthase V is dispensable for vesicle motility but required for virulence of the maize pathogen Ustilago maydis.
Article in The Plant cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- ZfpA-regulated chitin synthesis inAntimicrobial agents and chemotherapy · 2026Article
- Fungal Chitin Synthases: Structure, Function, and Regulation.Journal of fungi (Basel, Switzerland) · 2025Review
- Secretion and endocytosis in subapical cells support hyphal tip growth in the fungus Trichoderma reesei.Nature communications · 2025Article
- Fungal Pathogenesis-Related Cell Wall Biogenesis, with Emphasis on the Maize Anthracnose FungusPlants (Basel, Switzerland) · 2022Review
- Covering Soybean Leaves With Cellulose Nanofiber Changes Leaf Surface Hydrophobicity and Confers Resistance AgainstFrontiers in plant science · 2021Article
- Class V chitin synthase and β(1,3)-glucan synthase co-travel in the same vesicle in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2020Article
- Genomes and secretomes of Ascomycota fungi reveal diverse functions in plant biomass decomposition and pathogenesis.BMC genomics · 2019Article
- Off the wall: The rhyme and reason ofCell surface (Amsterdam, Netherlands) · 2019Review
- Members of chitin synthase family in Metarhizium acridum differentially affect fungal growth, stress tolerances, cell wall integrity and virulence.PLoS pathogens · 2019Article
- CRISPR/Cas9-mediated endogenous gene tagging in Fusarium oxysporum.Fungal genetics and biology : FG & B · 2019Article
- Sugar Partitioning betweenPlant physiology · 2019Article
- In-vitro Detection of Phytopathogenic Fungal Cell Wall by Polyclonal Sera Raised Against Trimethyl Chitosan Nanoparticles.International journal of nanomedicine · 2019Article
- The Myosin Motor Domain-Containing Chitin Synthases Are Involved in Cell Wall Integrity and Sensitivity to Antifungal Proteins inFrontiers in microbiology · 2019Article
- Cytoplasmic Transport Machinery of the SPF27 Homologue Num1 in Ustilago maydis.Scientific reports · 2018Article
- Fluorescent markers of various organelles in the wheat pathogen Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2017Article
- The Fungal Cell Wall: Structure, Biosynthesis, and Function.Microbiology spectrum · 2017Review
- Article
- Cell Biology of Hyphal Growth.Microbiology spectrum · 2017Review
- Co-delivery of cell-wall-forming enzymes in the same vesicle for coordinated fungal cell wall formation.Nature microbiology · 2016Article
- Active diffusion and microtubule-based transport oppose myosin forces to position organelles in cells.Nature communications · 2016Article
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Authors and funding
4 authors.
Funding
Abstract
Class V chitin synthases are fungal virulence factors required for plant infection. They consist of a myosin motor domain fused to a membrane-spanning chitin synthase region that participates in fungal cell wall formation. The function of the motor domain is unknown, but it might deliver the myosin chitin synthase-attached vesicles to the growth region. Here, we analyze the importance of both domains in Mcs1, the chitin synthase V of the maize smut fungus Ustilago maydis. By quantitative analysis of disease symptoms, tissue colonization, and single-cell morphogenic parameters, we demonstrate that both domains are required for fungal virulence. Fungi carrying mutations in the chitin synthase domain are rapidly recognized and killed by the plant, whereas fungi carrying a deletion of the motor domain show alterations in cell wall composition but can invade host tissue and cause a moderate plant response. We also show that Mcs1-bound vesicles exhibit long-range movement for up to 20 microm at a velocity of approximately 1.75 microm/s. Apical Mcs1 localization depends on F-actin and the motor domain, whereas Mcs1 motility requires microtubules and persists when the Mcs1 motor domain is deleted. Our results suggest that the myosin motor domain of ChsV supports exocytosis but not long-range delivery of transport vesicles.
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