ArticleCancer research2010
A cancer detection platform which measures telomerase activity from live circulating tumor cells captured on a microfilter.
Article in Cancer research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed.
- Circulating tumor cell telomerase activity as a prognostic marker for overall survival in SWOG 0421: a phase III metastatic castration resistant prostate cancer trial.International journal of cancer · 2015Trial
- Circulating tumor cell counts are prognostic of overall survival in SWOG S0421: a phase III trial of docetaxel with or without atrasentan for metastatic castration-resistant prostate cancer.Journal of clinical oncology : official journal of the American Society of Clinical Oncology · 2014Trial
- Immunomagnetic Isolation of HER2-Positive Breast Cancer Cells Using a Microfluidic Device.ACS omega · 2023Article
- Prognostic impact of circulating tumor cells detected with the microfluidic "universal CTC-chip" for primary lung cancer.Cancer science · 2022Article
- Integrated microdevice with a windmill-like hole array for the clog-free, efficient, and self-mixing enrichment of circulating tumor cells.Microsystems & nanoengineering · 2022Article
- Real-Time Detection of Tumor Cells during Capture on a Filter Element Significantly Enhancing Detection Rate.Biosensors · 2021Article
- Circulating Tumor Cells from Enumeration to Analysis: Current Challenges and Future Opportunities.Cancers · 2021Review
- Circulating Tumor Cell Detection Technologies and Clinical Utility: Challenges and Opportunities.Cancers · 2020Review
- Immunomagnetic separation of circulating tumor cells with microfluidic chips and their clinical applications.Biomicrofluidics · 2020Review
- Characterization of a novel automated microfiltration device for the efficient isolation and analysis of circulating tumor cells from clinical blood samples.Scientific reports · 2020Article
- Magnetic Cell Centrifuge Platform Performance Study with Different Microsieve Pore Geometries.Sensors (Basel, Switzerland) · 2019Article
- Hybrid microfluidic sorting of rare cells based on high throughput inertial focusing and high accuracy acoustic manipulation.RSC advances · 2019Article
- Fast and efficient microfluidic cell filter for isolation of circulating tumor cells from unprocessed whole blood of colorectal cancer patients.Scientific reports · 2019Observational
- Current status of liquid biopsies for the detection and management of prostate cancer.Cancer management and research · 2019Review
- Blood-based biopsies-clinical utility beyond circulating tumor cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2018Article
- Microfabrication of Micropore Array for Cell Separation and Cell Assay.Micromachines · 2018Article
- Development and Application of Liquid Biopsies in Metastatic Prostate Cancer.Current oncology reports · 2018Review
- 2.5-Dimensional Parylene C micropore array with a large area and a high porosity for high-throughput particle and cell separation.Microsystems & nanoengineering · 2018Article
- Treatment for non-small-cell lung cancer and circulating tumor cells.Lung cancer management · 2017Review
- Telomeres and telomerase in prostate cancer development and therapy.Nature reviews. Urology · 2017Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Circulating tumor cells (CTC) quantified in cancer patients' blood can predict disease outcome and response to therapy. However, the CTC analysis platforms commonly used cannot capture live CTCs and only apply to tumors of epithelial origin. To address these limitations, we have developed a novel cancer detection platform which measures telomerase activity from live CTCs captured on a parylene-C slot microfilter. Using a constant low-pressure delivery system, the new microfilter platform was capable of cell capture from 1 mL of whole blood in less than 5 minutes, achieving 90% capture efficiency, 90% cell viability, and 200-fold sample enrichment. Importantly, the captured cells retained normal morphology by scanning electron microscopy and could be readily manipulated, further analyzed, or expanded on- or off-filter. Telomerase activity--a well-recognized universal cancer marker--was reliably detected by quantitative PCR from as few as 25 cancer cells added into 7.5 mL of whole blood and captured on the microfilter. Moreover, significant telomerase activity elevation was also measured from patients' blood samples and from single cancer cells lifted off of the microfilter. Live CTC capture and analysis is fast and simple yet highly quantitative, versatile, and applicable to nearly all solid tumor types, making this a highly promising new strategy for cancer detection and characterization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.