ArticleDNA repair2010
A single-strand specific lesion drives MMS-induced hyper-mutability at a double-strand break in yeast.
Article in DNA repair, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 53 citations in OpenAlex.
- Increased replication-associated single-stranded DNA promotes formaldehyde-induced mutagenesis.bioRxiv : the preprint server for biology · 2026Article
- Translesion polymerases and DNA-protein crosslink repair shapes the cellular response to formaldehyde-induced DNA damage in ssDNA.Nucleic acids research · 2025Article
- Changes in metabolic landscapes shape divergent but distinct mutational signatures and cytotoxic consequences of redox stress.Nucleic acids research · 2023Article
- Acetaldehyde makes a distinct mutation signature in single-stranded DNA.Nucleic acids research · 2022Article
- The Shu complex prevents mutagenesis and cytotoxicity of single-strand specific alkylation lesions.eLife · 2021Article
- Balancing DNA repair to prevent ageing and cancer.Experimental cell research · 2021Review
- Article
- Mutation signatures specific to DNA alkylating agents in yeast and cancers.Nucleic acids research · 2020Article
- Rad52 Restrains Resection at DNA Double-Strand Break Ends in Yeast.Molecular cell · 2019Article
- Repair of base damage within break-induced replication intermediates promotes kataegis associated with chromosome rearrangements.Nucleic acids research · 2019Article
- Strand break-induced replication fork collapse leads to C-circles, C-overhangs and telomeric recombination.PLoS genetics · 2019Article
- Article
- Break-Induced Replication: The Where, The Why, and The How.Trends in genetics : TIG · 2018Review
- Eukaryotic DNA Polymerases in Homologous Recombination.Annual review of genetics · 2016Review
- The Shu complex promotes error-free tolerance of alkylation-induced base excision repair products.Nucleic acids research · 2016Article
- DNA Replication Stress Phosphoproteome Profiles Reveal Novel Functional Phosphorylation Sites on Xrs2 in Saccharomyces cerevisiae.Genetics · 2016Article
- Error-Prone Repair of DNA Double-Strand Breaks.Journal of cellular physiology · 2016Review
- Clusters of Multiple Mutations: Incidence and Molecular Mechanisms.Annual review of genetics · 2015Review
- The oxidative demethylase ALKBH3 marks hyperactive gene promoters in human cancer cells.Genome medicine · 2015Article
- Hypermutation in human cancer genomes: footprints and mechanisms.Nature reviews. Cancer · 2014Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Localized hyper-mutability (LHM) can be important in evolution, immunity, and genetic diseases. We previously reported that single-strand DNA (ssDNA) can be an important source of damage-induced LHM in yeast. Here, we establish that the generation of LHM by methyl methanesulfonate (MMS) during repair of a chromosomal double-strand break (DSB) can result in over 0.2 mutations/kb, which is approximately 20,000-fold higher than the MMS-induced mutation density without a DSB. The MMS-induced mutations associated with DSB repair were primarily due to substitutions via translesion DNA synthesis at damaged cytosines, even though there are nearly 10 times more MMS-induced lesions at other bases. Based on this mutation bias, the promutagenic lesion dominating LHM is likely 3-methylcytosine, which is single-strand specific. Thus, the dramatic increase in mutagenesis at a DSB is concluded to result primarily from the generation of non-repairable lesions in ssDNA associated with DSB repair along with efficient induction of highly mutagenic ssDNA-specific lesions. These findings with MMS-induced LHM have broad biological implications for unrepaired damage generated in ssDNA and possibly ssRNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.