ArticleMolecular microbiology2010
A two-component Kdo hydrolase in the inner membrane of Francisella novicida.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Lipopolysaccharide Recognition in the Crossroads of TLR4 and Caspase-4/11 Mediated Inflammatory Pathways.Frontiers in immunology · 2020Review
- Aminosugar-based immunomodulator lipid A: synthetic approaches.Beilstein journal of organic chemistry · 2018Review
- Molecular characterization and verification of azido-3,8-dideoxy-d-The Journal of biological chemistry · 2017Article
- Kdo2 -lipid A: structural diversity and impact on immunopharmacology.Biological reviews of the Cambridge Philosophical Society · 2015Review
- From the Outside-In: The Francisella tularensis Envelope and Virulence.Frontiers in cellular and infection microbiology · 2015Review
- Extragenic suppressor mutations in ΔripA disrupt stability and function of LpxA.BMC microbiology · 2014Article
- Synthesis of zwitterionic 1,1'-glycosylphosphodiester: a partial structure of galactosamine-modified Francisella lipid A.Organic letters · 2014Article
- Construction and characterization of an Escherichia coli mutant producing Kdo₂-lipid A.Marine drugs · 2014Article
- Metabolic labeling to characterize the overall composition of Francisella lipid A and LPS grown in broth and in human phagocytes.Innate immunity · 2014Article
- The atypical lipopolysaccharide of Francisella.Carbohydrate research · 2013Review
- Fortifying the barrier: the impact of lipid A remodelling on bacterial pathogenesis.Nature reviews. Microbiology · 2013Review
- Article
- Francisella is sensitive to insect antimicrobial peptides.Journal of innate immunity · 2013Article
- NaxD is a deacetylase required for lipid A modification and Francisella pathogenesis.Molecular microbiology · 2012Article
- Subversion of host recognition and defense systems by Francisella spp.Microbiology and molecular biology reviews : MMBR · 2012Review
- The lipopolysaccharide from Capnocytophaga canimorsus reveals an unexpected role of the core-oligosaccharide in MD-2 binding.PLoS pathogens · 2012Article
- A typical preparation of Francisella tularensis O-antigen yields a mixture of three types of saccharides.Biochemistry · 2011Article
- 3-Deoxy-D-manno-octulosonic acid (Kdo) hydrolase identified in Francisella tularensis, Helicobacter pylori, and Legionella pneumophila.The Journal of biological chemistry · 2010Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Lipid A coats the outer surface of the outer membrane of Gram-negative bacteria. In Francisella tularensis subspecies novicida lipid A is present either as the covalently attached anchor of lipopolysaccharide (LPS) or as free lipid A. The lipid A moiety of Francisella LPS is linked to the core domain by a single 2-keto-3-deoxy-D-manno-octulosonic acid (Kdo) residue. F. novicida KdtA is bi-functional, but F. novicida contains a membrane-bound Kdo hydrolase that removes the outer Kdo unit. The hydrolase consists of two proteins (KdoH1 and KdoH2), which are expressed from adjacent, co-transcribed genes. KdoH1 (related to sialidases) has a single predicted N-terminal transmembrane segment. KdoH2 contains 7 putative transmembrane sequences. Neither protein alone catalyses Kdo cleavage when expressed in E. coli. Activity requires simultaneous expression of both proteins or mixing of membranes from strains expressing the individual proteins under in vitro assay conditions in the presence of non-ionic detergent. In E. coli expressing KdoH1 and KdoH2, hydrolase activity is localized in the inner membrane. WBB06, a heptose-deficient E. coli mutant that makes Kdo(2) -lipid A as its sole LPS, accumulates Kdo-lipid A when expressing the both hydrolase components, and 1-dephospho-Kdo-lipid A when expressing both the hydrolase and the Francisella lipid A 1-phosphatase (LpxE).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.