ArticleBiomedical microdevices2010
A low resistance microfluidic system for the creation of stable concentration gradients in a defined 3D microenvironment.
Article in Biomedical microdevices, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed.
- An Insight on Microfluidic Organ-on-a-Chip Models for PMACS omega · 2024Review
- Gradient to sectioning CUBE workflow for the generation and imaging of organoids with localized differentiation.Communications biology · 2023Article
- Proline and glucose metabolic reprogramming supports vascular endothelial and medial biomass in pulmonary arterial hypertension.JCI insight · 2023Article
- A Pillar-Free Diffusion Device for Studying Chemotaxis on Supported Lipid Bilayers.Micromachines · 2021Article
- Flow-assembled chitosan membranes in microfluidics: recent advances and applications.Journal of materials chemistry. B · 2021Review
- Tuning the porosity of biofabricated chitosan membranes in microfluidics with co-assembled nanoparticles as templates.Materials advances · 2020Article
- Article
- A simple and reusable bilayer membrane-based microfluidic device for the study of gradient-mediated bacterial behaviors.Biomicrofluidics · 2017Article
- Development-on-chip: in vitro neural tube patterning with a microfluidic device.Development (Cambridge, England) · 2016Article
- Simultaneous or Sequential Orthogonal Gradient Formation in a 3D Cell Culture Microfluidic Platform.Small (Weinheim an der Bergstrasse, Germany) · 2016Article
- Design of a microfluidic device to quantify dynamic intra-nuclear deformation during cell migration through confining environments.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Microfluidic 3D models of cancer.Advanced drug delivery reviews · 2014Review
- A sensitive chemotaxis assay using a novel microfluidic device.BioMed research international · 2013Article
- Chemistry with spatial control using particles and streams().RSC advances · 2012Article
- Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels.Nature protocols · 2012Article
- Transport and shear in a microfluidic membrane bilayer device for cell culture.Biomicrofluidics · 2011Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The advent of microfluidic technology allows control and interrogation of cell behavior by defining the local microenvironment with an assortment of biochemical and biophysical stimuli. Many approaches have been developed to create gradients of soluble factors, but the complexity of such systems or their inability to create defined and controllable chemical gradients has limited their widespread implementation. Here we describe a new microfluidic device which employs a parallel arrangement of wells and channels to create stable, linear concentration gradients in a gel region between a source and a sink well. Pressure gradients between the source and sink wells are dissipated through low resistance channels in parallel with the gel channel, thus minimizing the convection of solute in this region. We demonstrate the ability of the new device to quantitate chemotactic responses in a variety of cell types, yielding a complete profile of the migratory response and representing the total number of migrating cells and the distance each cell has migrated. Additionally we show the effect of concentration gradients of the morphogen Sonic hedgehog on the specification of differentiating neural progenitors in a 3-dimensional matrix.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.