ArticlePLoS genetics2010
Inter- and intra-individual variation in allele-specific DNA methylation and gene expression in children conceived using assisted reproductive technology.
Article in PLoS genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
73 citing papers in PubMed, 2 syntheses or guidelines pooled it, 168 citations in OpenAlex.
- A systematic review of genome-wide analyses of methylation changes associated with assisted reproductive technologies in various tissues.Fertility and sterility · 2024Pooled it
- Risk of adverse pregnancy and perinatal outcomes after high technology infertility treatment: a comprehensive systematic review.Reproductive biology and endocrinology : RB&E · 2016Pooled it
- Comparison of DNA methylation patterns of parentally imprinted genes in placenta derived from IVF conceptions in two different culture media.Human reproduction (Oxford, England) · 2020Trial
- Interindividual epigenetic variation in ABCB1 promoter and its relationship with ABCB1 expression and function in healthy Chinese subjects.British journal of clinical pharmacology · 2015Trial
- Abnormal epigenetic aging of epigenetic outliers in normal colon mucosa from colorectal cancer patients.Scientific reports · 2026Article
- Assessing the impact of medically assisted reproduction on autism spectrum disorder risk.Journal of assisted reproduction and genetics · 2024Article
- Mitochondrial DNA Deficiency and Supplementation inInternational journal of molecular sciences · 2023Article
- Metabolomic Profile of Children Conceived With Medically Assisted Technologies.The Journal of clinical endocrinology and metabolism · 2023Article
- Embryo cryopreservation leads to sex-specific DNA methylation perturbations in both human and mouse placentas.Human molecular genetics · 2022Article
- Parental Programming of Offspring Health: The Intricate Interplay between Diet, Environment, Reproduction and Development.Biomolecules · 2022Review
- DNA Methylation in Offspring Conceived after Assisted Reproductive Techniques: A Systematic Review and Meta-Analysis.Journal of clinical medicine · 2022Review
- Methylome-wide analysis of IVF neonates that underwent embryo culture in different media revealed no significant differences.NPJ genomic medicine · 2022Article
- Contemporary Use of ICSI and Epigenetic Risks to Future Generations.Journal of clinical medicine · 2022Review
- The Consequences of Assisted Reproduction Technologies on the Offspring Health Throughout Life: A Placental Contribution.Frontiers in cell and developmental biology · 2022Review
- Epigenetic Modifications at the Center of the Barker Hypothesis and Their Transgenerational Implications.International journal of environmental research and public health · 2021Review
- Genetic and in utero environmental contributions to DNA methylation variation in placenta.Human molecular genetics · 2021Article
- The effects of assisted reproduction technologies on metabolic health and disease†.Biology of reproduction · 2021Review
- Reproductive fluids, used for the in vitro production of pig embryos, result in healthy offspring and avoid aberrant placental expression of PEG3 and LUM.Journal of animal science and biotechnology · 2021Article
- Can Time-Lapse Incubation and Monitoring Be Beneficial to Assisted Reproduction Technology Outcomes? A Randomized Controlled Trial Using Day 3 Double Embryo Transfer.Frontiers in physiology · 2021Article
- The hypomethylation of imprinted genes in IVF/ICSI placenta samples is associated with concomitant changes in histone modifications.Epigenetics · 2020Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Epidemiological studies have reported a higher incidence of rare disorders involving imprinted genes among children conceived using assisted reproductive technology (ART), suggesting that ART procedures may be disruptive to imprinted gene methylation patterns. We examined intra- and inter-individual variation in DNA methylation at the differentially methylated regions (DMRs) of the IGF2/H19 and IGF2R loci in a population of children conceived in vitro or in vivo. We found substantial variation in allele-specific methylation at both loci in both groups. Aberrant methylation of the maternal IGF2/H19 DMR was more common in the in vitro group, and the overall variance was also significantly greater in the in vitro group. We estimated the number of trophoblast stem cells in each group based on approximation of the variance of the binomial distribution of IGF2/H19 methylation ratios, as well as the distribution of X chromosome inactivation scores in placenta. Both of these independent measures indicated that placentas of the in vitro group were derived from fewer stem cells than the in vivo conceived group. Both IGF2 and H19 mRNAs were significantly lower in placenta from the in vitro group. Although average birth weight was lower in the in vitro group, we found no correlation between birth weight and IGF2 or IGF2R transcript levels or the ratio of IGF2/IGF2R transcript levels. Our results show that in vitro conception is associated with aberrant methylation patterns at the IGF2/H19 locus. However, very little of the inter- or intra-individual variation in H19 or IGF2 mRNA levels can be explained by differences in maternal DMR DNA methylation, in contrast to the expectations of current transcriptional imprinting models. Extraembryonic tissues of embryos cultured in vitro appear to be derived from fewer trophoblast stem cells. It is possible that this developmental difference has an effect on placental and fetal growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.