ArticleThe Journal of general physiology2010
Recording single-channel activity of inositol trisphosphate receptors in intact cells with a microscope, not a patch clamp.
Article in The Journal of general physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 57 citations in OpenAlex.
- Visualizing PIEZO1 localization and activity in hiPSC-derived single cells and organoids with HaloTag technology.Nature communications · 2025Article
- Biological physics to uncover cell signaling.Biophysical reviews · 2025Review
- Visualizing PIEZO1 Localization and Activity in hiPSC-Derived Single Cells and Organoids with HaloTag Technology.bioRxiv : the preprint server for biology · 2025Article
- Analysis of Store-Operated CaMethods in molecular biology (Clifton, N.J.) · 2025Article
- Dissociation of inositol 1,4,5-trisphosphate from IPThe Journal of biological chemistry · 2023Article
- Physiological levels of fluid shear stress modulate vascular function through TRPV4 sparklets.Acta biochimica et biophysica Sinica · 2022Article
- Termination of CaCell calcium · 2021Article
- KRAP tethers IPNature communications · 2021Article
- High-throughput single-cell live imaging of photobiomodulation with multispectral near-infrared lasers in cultured T cells.Journal of biomedical optics · 2020Article
- Spatial-temporal patterning of CaSeminars in cell & developmental biology · 2019Review
- Structure and Function of IPCold Spring Harbor perspectives in biology · 2019Review
- Calcium signalling in T cells.Nature reviews. Immunology · 2019Review
- Spatially structured cell populations process multiple sensory signals in parallel in intact vascular endothelium.Science signaling · 2018Article
- All three IPScience signaling · 2018Article
- All three IPJournal of cell science · 2018Article
- Dynamic CaCell calcium · 2018Article
- Nitric Oxide-Dependent Feedback Loop Regulates Transient Receptor Potential Vanilloid 4 (TRPV4) Channel Cooperativity and Endothelial Function in Small Pulmonary Arteries.Journal of the American Heart Association · 2017Article
- CaNature communications · 2017Article
- Endogenous signalling pathways and caged IPJournal of cell science · 2017Article
- Real-Time Sensing of Single-Ligand Delivery with Nanoaperture-Integrated Microfluidic Devices.ACS omega · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Optical single-channel recording is a novel tool for the study of individual Ca2+-permeable channels within intact cells under minimally perturbed physiological conditions. As applied to the functioning and spatial organization of IP3Rs, this approach complements our existing knowledge, which derives largely from reduced systems - such as reconstitution into lipid bilayers and patch clamping of IP3Rs on the membrane of excised nuclei - where the spatial arrangement and interactions among IP3Rs via CICR are disrupted. The ability to image the activity of single IP3R channels with millisecond resolution together with localization of their positions with a precision of a few tens of nanometers both raises several intriguing questions and holds promise of answers. In particular, what mechanism underlies the anchoring of puffs and blips to static locations; why do these Ca2+ release events appear to involve only a very small fraction of the IP3Rs within a cell; and how can we reconcile the relative immotility of functional IP3Rs with numerous studies reporting free diffusion of IP3R protein in the ER membrane?
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.