ArticleThe Journal of experimental medicine2010
Anaplasma phagocytophilum induces actin phosphorylation to selectively regulate gene transcription in Ixodes scapularis ticks.
Article in The Journal of experimental medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 66 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
66 citing papers in PubMed, 86 citations in OpenAlex.
- Obligatory intracellular bacterium Anaplasma phagocytophilum AnkA regulates actin dynamics and spatiotemporal bacterial release.PLoS pathogens · 2026Article
- Coinfection ecology and pathogen emergence in aApplied and environmental microbiology · 2026Article
- Arthropod exosomal glycine-rich protein as a potential vaccine candidate effectively reduces tick blood-feeding and pathogen transmission.The EMBO journal · 2026Article
- Coinfection Ecology and Pathogen Emergence in amedRxiv : the preprint server for health sciences · 2025Article
- An arthropod transporter IsOATP4056 exerts inhibitory effect on its interacting membrane protein to facilitate rickettsial pathogen survival in ticks.Cell communication and signaling : CCS · 2025Article
- Arthropod autophagy molecules facilitate Anaplasma phagocytophilum infection of Ixodes scapularis tick cells.Communications biology · 2025Article
- Article
- Transcriptomic analysis of Rhipicephalus microplus hemocytes from female ticks infected with Babesia bovis or Babesia bigemina.Parasites & vectors · 2025Article
- Use of Tick Cell Lines in Co-Infection Studies with a Preliminary Study of Co-Culture ofPathogens (Basel, Switzerland) · 2025Article
- Analysis ofbioRxiv : the preprint server for biology · 2024Article
- Antibody-blocking of a tick transporter impairs Anaplasma phagocytophilum colonization in Haemaphysalis longicornis ticks.Scientific reports · 2024Article
- How mesenchymal stem cells transform into adipocytes: Overview of the current understanding of adipogenic differentiation.World journal of stem cells · 2024Review
- Analysis ofFrontiers in cellular and infection microbiology · 2024Article
- Immunization against arthropod protein impairs transmission of rickettsial pathogen from ticks to the vertebrate host.NPJ vaccines · 2023Article
- Enhancement of Cell Adhesion byJournal of personalized medicine · 2023Article
- Rickettsial pathogen inhibits tick cell death through tryptophan metabolite mediated activation of p38 MAP kinase.iScience · 2023Article
- Identification of microRNAs in the Lyme Disease VectorInternational journal of molecular sciences · 2022Article
- Rickettsial Pathogen Perturbs Tick Circadian Gene to Infect the Vertebrate Host.International journal of molecular sciences · 2022Article
- Review
- Double anus in an Ixodes scapularis nymph, a medically important tick vector.Parasites & vectors · 2021Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
Anaplasma phagocytophilum, the agent of human anaplasmosis, persists in ticks and mammals. We show that A. phagocytophilum induces the phosphorylation of actin in an Ixodes ricinus tick cell line and Ixodes scapularis ticks, to alter the ratio of monomeric/filamentous (G/F) actin. A. phagocytophilum-induced actin phosphorylation was dependent on Ixodes p21-activated kinase (IPAK1)-mediated signaling. A. phagocytophilum stimulated IPAK1 activity via the G protein-coupled receptor Gbetagamma subunits, which mediated phosphoinositide 3-kinase (PI3K) activation. Disruption of Ixodes gbetagamma, pi3k, and pak1 reduced actin phosphorylation and bacterial acquisition by ticks. A. phagocytophilum-induced actin phosphorylation resulted in increased nuclear G actin and phosphorylated actin. The latter, in association with RNA polymerase II (RNAPII), enhanced binding of TATA box-binding protein to RNAPII and selectively promoted expression of salp16, a gene crucial for A. phagocytophilum survival. These data define a mechanism that A. phagocytophilum uses to selectively alter arthropod gene expression for its benefit and suggest new strategies to interfere with the life cycle of this intracellular pathogen, and perhaps other Rickettsia-related microbes of medical importance.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.