ArticleNucleic acids research2010
Genetic and functional interactions between Mus81-Mms4 and Rad27.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- A Rfa1-MN-based system reveals new factors involved in the rescue of broken replication forks.PLoS genetics · 2025Article
- The secondary-structured DNA-binding activity of Dna2 endonuclease/helicase is critical to cell growth under replication stress.The FEBS journal · 2021Article
- Review
- Complex repeat structure promotes hyper-amplification and amplicon evolution through rolling-circle replication.Nucleic acids research · 2018Article
- The possible function of Flp1 in homologous recombination repair inAIMS genetics · 2018Article
- Control of structure-specific endonucleases to maintain genome stability.Nature reviews. Molecular cell biology · 2017Review
- Srs2 promotes Mus81-Mms4-mediated resolution of recombination intermediates.Nucleic acids research · 2015Article
- The DNA repair endonuclease Mus81 facilitates fast DNA replication in the absence of exogenous damage.Nature communications · 2015Article
- A physiological significance of the functional interaction between Mus81 and Rad27 in homologous recombination repair.Nucleic acids research · 2015Article
- Hold your horSSEs: controlling structure-selective endonucleases MUS81 and Yen1/GEN1.Frontiers in genetics · 2015Review
- Rad52/Rad59-dependent recombination as a means to rectify faulty Okazaki fragment processing.The Journal of biological chemistry · 2014Article
- Human MUS81-EME2 can cleave a variety of DNA structures including intact Holliday junction and nicked duplex.Nucleic acids research · 2014Article
- A saccharomyces cerevisiae RNase H2 interaction network functions to suppress genome instability.Molecular and cellular biology · 2014Article
- A winged helix domain in human MUS81 binds DNA and modulates the endonuclease activity of MUS81 complexes.Nucleic acids research · 2013Article
- Premature Cdk1/Cdc5/Mus81 pathway activation induces aberrant replication and deleterious crossover.The EMBO journal · 2013Article
- Article
- R-loops and nicks initiate DNA breakage and genome instability in non-growing Escherichia coli.Nature communications · 2013Article
- Sequence, structure and functional diversity of PD-(D/E)XK phosphodiesterase superfamily.Nucleic acids research · 2012Article
- The trans-autostimulatory activity of Rad27 suppresses dna2 defects in Okazaki fragment processing.The Journal of biological chemistry · 2012Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The two endonucleases, Rad27 (yeast Fen1) and Dna2, jointly participate in the processing of Okazaki fragments in yeasts. Mus81-Mms4 is a structure-specific endonuclease that can resolve stalled replication forks as well as toxic recombination intermediates. In this study, we show that Mus81-Mms4 can suppress dna2 mutational defects by virtue of its functional and physical interaction with Rad27. Mus81-Mms4 stimulated Rad27 activity significantly, accounting for its ability to restore the growth defects caused by the dna2 mutation. Interestingly, Rad27 stimulated the rate of Mus81-Mms4 catalyzed cleavage of various substrates, including regressed replication fork substrates. The ability of Rad27 to stimulate Mus81-Mms4 did not depend on the catalytic activity of Rad27, but required the C-terminal 64 amino acid fragment of Rad27. This indicates that the stimulation was mediated by a specific protein-protein interaction between the two proteins. Our in vitro data indicate that Mus81-Mms4 and Rad27 act together during DNA replication and resolve various structures that can impede normal DNA replication. This conclusion was further strengthened by the fact that rad27 mus81 or rad27 mms4 double mutants were synergistically lethal. We discuss the significance of the interactions between Rad27, Dna2 and Mus81-Mms4 in context of DNA replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.