ArticleNucleic acids research2010
NMR structure of the let-7 miRNA interacting with the site LCS1 of lin-41 mRNA from Caenorhabditis elegans.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 29 citations in OpenAlex.
- Effect of Internal and Bulge Loops on the Thermal Stability of Small DNA Duplexes.The journal of physical chemistry. B · 2024Article
- miRNA-Based Regulation of Alternative RNA Splicing in Metazoans.International journal of molecular sciences · 2021Review
- DNA Satellites Are Transcribed as Part of the Non-Coding Genome in Eukaryotes and Bacteria.Genes · 2021Article
- Recent Molecular Genetic Explorations ofGenetics · 2018Article
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- Functional relevance of "seed" and "non-seed" sequences in microRNA-mediated promotion of C. elegans developmental progression.RNA (New York, N.Y.) · 2015Article
- High resolution 4D HPCH experiment for sequential assignment of (13)C-labeled RNAs via phosphodiester backbone.Journal of biomolecular NMR · 2015Article
- miRNAs as Biomarkers in Chronic Myelogenous Leukemia.Drug development research · 2015Review
- Thermodynamic examination of 1- to 5-nt purine bulge loops in RNA and DNA constructs.RNA (New York, N.Y.) · 2015Article
- Structural features of a 3' splice site in influenza a.Biochemistry · 2015Article
- Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay.Journal of visualized experiments : JoVE · 2015Article
- C4'/H4' selective, non-uniformly sampled 4D HC(P)CH experiment for sequential assignments of (13)C-labeled RNAs.Journal of biomolecular NMR · 2014Article
- 3'LIFE: a functional assay to detect miRNA targets in high-throughput.Nucleic acids research · 2014Article
- 4D Non-uniformly sampled C,C-NOESY experiment for sequential assignment of 13C, 15N-labeled RNAs.Journal of biomolecular NMR · 2013Article
- Tertiary structure-based analysis of microRNA-target interactions.RNA (New York, N.Y.) · 2013Article
- Stability of single-nucleotide bulge loops embedded in a GAAA RNA hairpin stem.RNA (New York, N.Y.) · 2012Article
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
We have determined the 3D structure of a 34-nt RNA construct, herein named LCS1co, which mimics the interaction of let-7 microRNA (miRNA) to one of its complementary binding sites, LCS1, in the 3'-untranslated region of lin-41 mRNA by solution-state NMR spectroscopy. let-7 miRNAs control the timing of development of the nematode Caenorhabditis elegans and are highly conserved in mammals. The sequence and structure of the two conserved let-7 complementary sites, LCS1 and LCS2, in the 3'-untranslated region of lin-41 mRNA are important for a proper downregulation of lin-41. The high-resolution NMR structure reveals details of the binding of let-7 miRNA to lin-41 mRNA which involves formation of a complex with non-canonical structural elements within the seed region. LCS1co exhibits a stem-loop structure with two stems, an asymmetric internal loop and an adenine bulge. Comparison with the NMR solution-state structure of the let-7:lin-41 complex involving the LCS2-binding site shows that conformational freedom of the asymmetric internal loop of LCS1co correlates with a smaller bend between the upper and lower stems in comparison to the well-defined asymmetric loop of LCS2co.
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