ArticleJournal of clinical microbiology2010
Specific, sensitive, and rapid diagnosis of active toxoplasmosis by a loop-mediated isothermal amplification method using blood samples from patients.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 1 of them a synthesis that pooled it.
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Who cites it
44 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Test accuracy of loop-mediated isothermal amplification for schistosomiasis in low endemicity areas: a systematic review and meta-analysis.Infectious diseases of poverty · 2025Pooled it
- A Systematic Review and Meta-Analysis of Diagnostic Test Accuracy for Human Toxoplasmosis: Performance, Populations, and Validation Gaps.Medical devices (Auckland, N.Z.) · 2026Review
- Comprehensive diagnostic approaches to feline toxoplasmosis: Bridging traditional methods and emerging technologies.Virulence · 2025Review
- A novel single-tube LAMP-CRISPR/Cas12b method for rapid and visual detection of zoonotic Toxoplasma gondii in the environment.Infectious diseases of poverty · 2024Article
- Trend in serological and molecular diagnostic methods for Toxoplasma gondii infection.European journal of medical research · 2024Review
- Development of a LAMP method with lateral flow DNA chromatography to diagnose toxoplasmosis in immunocompromised patients.Tropical medicine and health · 2024Article
- Molecular detection of toxoplasmosis in wild rats using loop-mediated isothermal amplification assay.Veterinary world · 2024Article
- Seroprevalence and Genetic Characterization ofJournal of tropical medicine · 2022Article
- Clinical validation of visual LAMP and qLAMP assays for the rapid detection ofFrontiers in cellular and infection microbiology · 2022Article
- One-Day Molecular Detection ofFoods (Basel, Switzerland) · 2021Article
- Loop-mediated isothermal amplification (LAMP) assay for detection of sesame phyllody phytoplasmas in Vietnam.Folia microbiologica · 2021Article
- The Loop-Mediated Isothermal Amplification Technique in Periodontal Diagnostics: A Systematic Review.Journal of clinical medicine · 2021Review
- Development and validation of a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of ZIKV in patient samples from Brazil.Scientific reports · 2021Article
- Optimization of a Loop-Mediated Isothermal Amplification Assay as a Point-of-Care Tool for the Detection ofJournal of parasitology research · 2021Article
- A Review on the Prevalence ofInternational journal of environmental research and public health · 2020Review
- Validation of Loop-Mediated Isothermal Amplification (LAMP) Field Tool for Rapid and Sensitive Diagnosis of Contagious Agalactia in Small Ruminants.Animals : an open access journal from MDPI · 2020Article
- Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Review
- Isothermal PCR for Feasible Molecular Diagnosis of Primary Toxoplasmosis in Women Recently Experienced Spontaneous Abortion.Open access Macedonian journal of medical sciences · 2018Article
- Advances in serological, imaging techniques and molecular diagnosis of Toxoplasma gondii infection.Infection · 2018Review
- Visual loop-mediated isothermal amplification (LAMP) for the rapid diagnosis of Enterocytozoon hepatopenaei (EHP) infection.Parasitology research · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Loop-mediated isothermal amplification (LAMP), a rapid nucleic acid amplification method, was developed for the clinical diagnosis of toxoplasmosis. Three LAMP assays based on the SAG1, SAG2, and B1 genes of Toxoplasma gondii were developed. The sensitivities and specificities of the LAMP assays were evaluated by comparison with the results of conventional nested PCR. The LAMP assays were highly sensitive and had a detection limit of 0.1 tachyzoite, and no cross-reactivity with the DNA of other parasites was observed. Blood was collected from 105 individuals to test the LAMP assays: 40 patients with active toxoplasmosis, 40 negative controls, and 25 patients with other parasitic infections. The SAG2-based LAMP (SAG2-LAMP) had a greater sensitivity (87.5%) than the SAG1-LAMP (80%), B1-LAMP (80%), and nested PCR (62.5%). All the LAMP assays and nested PCR were 100% specific. This is the first report of a study which applied the LAMP method to diagnose toxoplasmosis from human blood samples. Due to its simplicity, sensitivity, and specificity, LAMP is suggested as an appropriate method for routine diagnosis of active toxoplasmosis in humans.
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