ArticleClinical and vaccine immunology : CVI2010
Anthrax spore detection by a luminex assay based on monoclonal antibodies that recognize anthrose-containing oligosaccharides.
Article in Clinical and vaccine immunology : CVI, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
- Anthrax: A narrative review.New microbes and new infections · 2024Review
- Human Anthrax: Update of the Diagnosis and Treatment.Diagnostics (Basel, Switzerland) · 2023Review
- A CRISPR/Cas12a-based DNAzyme visualization system for rapid, non-electrically dependent detection ofEmerging microbes & infections · 2022Article
- Rapid and Sensitive Multiplex Assay for the Detection ofPathogens (Basel, Switzerland) · 2020Article
- Detection and Identification ofMicroorganisms · 2020Review
- Simultaneous Immunodetection of Anthrax, Plague, and Tularemia from Blood Cultures by Use of Multiplexed Suspension Arrays.Journal of clinical microbiology · 2018Article
- Impact of Aerosol Dust on xMAP Multiplex Detection of Different Class Pathogens.Frontiers in microbiology · 2017Article
- Glycosylation of BclA Glycoprotein from Bacillus cereus and Bacillus anthracis Exosporium Is Domain-specific.The Journal of biological chemistry · 2016Article
- Development of a bead-based Luminex assay using lipopolysaccharide specific monoclonal antibodies to detect biological threats from Brucella species.BMC microbiology · 2015Article
- Identification and characterization of glycoproteins on the spore surface of Clostridium difficile.Journal of bacteriology · 2014Article
- Article
- Quantitative and sensitive RNA based detection of Bacillus spores.Frontiers in microbiology · 2014Article
- De novo asymmetric synthesis of oligo-rhamno di- and tri-saccharides related to the anthrax tetrasaccharide.Tetrahedron · 2013Article
- Phage display-derived binders able to distinguish Listeria monocytogenes from other Listeria species.PloS one · 2013Article
- Article
- Fast and sensitive detection of Bacillus anthracis spores by immunoassay.Applied and environmental microbiology · 2012Article
- Identification of an African Bacillus anthracis lineage that lacks expression of the spore surface-associated anthrose-containing oligosaccharide.Journal of bacteriology · 2011Article
- Same-day detection of Escherichia coli O157:H7 from spinach by using electrochemiluminescent and cytometric bead array biosensors.Applied and environmental microbiology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The similarity of endospore surface antigens between bacteria of the Bacillus cereus group complicates the development of selective antibody-based anthrax detection systems. The surface of B. anthracis endospores exposes a tetrasaccharide containing the monosaccharide anthrose. Anti-tetrasaccharide monoclonal antibodies (MAbs) and anti-anthrose-rhamnose disaccharide MAbs were produced and tested for their fine specificities in a direct spore enzyme-linked immunosorbent assay (ELISA) with inactivated spores of a broad spectrum of B. anthracis strains and related species of the Bacillus genus. Although the two sets of MAbs had different fine specificities, all of them recognized the tested B. anthracis strains and showed only a limited cross-reactivity with two B. cereus strains. The MAbs were further tested for their ability to be implemented in a highly sensitive and specific bead-based Luminex assay. This assay detected spores from different B. anthracis strains and two cross-reactive B. cereus strains, correlating with the results obtained in direct spore ELISA. The Luminex assay (detection limit 10(3) to 10(4) spores per ml) was much more sensitive than the corresponding sandwich ELISA. Although not strictly specific for B. anthracis spores, the developed Luminex assay represents a useful first-line screening tool for the detection of B. anthracis spores.
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