ArticleThe Journal of biological chemistry2010
ATP-dependent pre-replicative complex assembly is facilitated by Adk1p in budding yeast.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Isobavachalcone effectively inhibits the growth ofAntimicrobial agents and chemotherapy · 2026Article
- Elucidation and engineering mitochondrial respiratory-related genes for improving bioethanol production at high temperature inEngineering microbiology · 2024Article
- Article
- Mitotic entry in the presence of DNA damage is a widespread property of aneuploidy in yeast.Molecular biology of the cell · 2015Article
- RB in breast cancer: differential effects in estrogen receptor-positive and estrogen receptor-negative disease.Cell cycle (Georgetown, Tex.) · 2010Article
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Authors and funding
6 authors.
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Abstract
Pre-replicative complex (pre-RC) assembly is a critical part of the mechanism that controls the initiation of DNA replication, and ATP binding and hydrolysis by multiple pre-RC proteins are essential for pre-RC assembly and activation. Here, we demonstrate that Adk1p (adenylate kinase 1 protein) plays an important role in pre-RC assembly in Saccharomyces cerevisiae. Isolated from a genetic screen, adk1(G20S) cells with a mutation within the nucleotide-binding site were defective in replication initiation. adk1Δ cells were viable at 25 °C but not at 37°C. Flow cytometry indicated that both the adk1-td (temperature-inducible degron) and adk1(G20S) mutants were defective in S phase entry. Furthermore, Adk1p bound to chromatin throughout the cell cycle and physically interacted with Orc3p, whereas the Adk1(G20S) protein had a reduced ability to bind chromatin and Orc3p without affecting the cellular ATP level. In addition, Adk1p associated with replication origins by ChIP assay. Finally, Adk1-td protein depletion prevented pre-RC assembly during the M-to-G(1) transition. We suggest that Adk1p regulates ATP metabolism on pre-RC proteins to promote pre-RC assembly and activation.
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