ArticleThe Journal of biological chemistry2010
The Arabidopsis peroxisomal ABC transporter, comatose, complements the Saccharomyces cerevisiae pxa1 pxa2Delta mutant for metabolism of long-chain fatty acids and exhibits fatty acyl-CoA-stimulated ATPase activity.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 73 citations in OpenAlex.
- Structural insights into Arabidopsis thaliana CTS-mediated fatty acid and hormone metabolism.Nature communications · 2026Article
- Predictive modelling of the COMATOSE transporter reveals a conserved ligand binding pocket for acyl-CoAs.Scientific reports · 2026Article
- Regulatory Role of Jasmonate Signaling in Dark-, Drought-, and Salt-Induced Leaf Senescence.International journal of molecular sciences · 2026Review
- A Structural Bioinformatics-Guided Study of Adenosine Triphosphate-Binding Cassette (ABC) Transporters and Their Substrates.Membranes · 2025Article
- 2021 update on ATP-binding cassette (ABC) transporters: how they meet the needs of plants.Plant physiology · 2021Review
- Jasmonic Acid Signaling and Molecular Crosstalk with Other Phytohormones.International journal of molecular sciences · 2021Review
- Long chain acyl CoA synthetase 4 catalyzes the first step in peroxisomal indole-3-butyric acid to IAA conversion.Plant physiology · 2021Article
- Acyl-CoA thioesterase activity of peroxisomal ABC protein ABCD1 is required for the transport of very long-chain acyl-CoA into peroxisomes.Scientific reports · 2021Article
- Peroxisomes: A New Hub for Metabolic Engineering in Yeast.Frontiers in bioengineering and biotechnology · 2021Article
- Peroxisomes form intralumenal vesicles with roles in fatty acid catabolism and protein compartmentalization in Arabidopsis.Nature communications · 2020Article
- Failure to Maintain Acetate Homeostasis by Acetate-Activating Enzymes Impacts Plant Development.Plant physiology · 2020Article
- PheophorbidePlant physiology · 2020Article
- ABCG1 contributes to suberin formation in Arabidopsis thaliana roots.Scientific reports · 2019Article
- Metabolic Alterations in the Enoyl-CoA Hydratase 2 Mutant Disrupt Peroxisomal Pathways in Seedlings.Plant physiology · 2019Article
- Mutagenesis separates ATPase and thioesterase activities of the peroxisomal ABC transporter, Comatose.Scientific reports · 2019Article
- Plant peroxisomal solute transporter proteins.Journal of integrative plant biology · 2019Review
- JASSY, a chloroplast outer membrane protein required for jasmonate biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Towards Identification of the Substrates of ATP-Binding Cassette Transporters.Plant physiology · 2018Review
- AProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Peroxisome Function, Biogenesis, and Dynamics in Plants.Plant physiology · 2018Review
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
Abstract
The Arabidopsis ABC transporter Comatose (CTS; AtABCD1) is required for uptake into the peroxisome of a wide range of substrates for β-oxidation, but it is uncertain whether CTS itself is the transporter or if the transported substrates are free acids or CoA esters. To establish a system for its biochemical analysis, CTS was expressed in Saccharomyces cerevisiae. The plant protein was correctly targeted to yeast peroxisomes, was assembled into the membrane with its nucleotide binding domains in the cytosol, and exhibited basal ATPase activity that was sensitive to aluminum fluoride and abrogated by mutation of a conserved Walker A motif lysine residue. The yeast pxa1 pxa2Δ mutant lacks the homologous peroxisomal ABC transporter and is unable to grow on oleic acid. Consistent with its exhibiting a function in yeast akin to that in the plant, CTS rescued the oleate growth phenotype of the pxa1 pxa2Δ mutant, and restored β-oxidation of fatty acids with a range of chain lengths and varying degrees of desaturation. When expressed in yeast peroxisomal membranes, the basal ATPase activity of CTS could be stimulated by fatty acyl-CoAs but not by fatty acids. The implications of these findings for the function and substrate specificity of CTS are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.