ArticleDevelopmental biology2010
Regulation of Dpp activity by tissue-specific cleavage of an upstream site within the prodomain.
Article in Developmental biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- TUSC3 serves as a rate-limiting gatekeeper of a glycan-mediated ER triage checkpoint for BMP4/Dpp.Cell reports · 2026Article
- TUSC3 serves as a rate-limiting gatekeeper of a glycan-mediated ER Triage Checkpoint for BMP4/Dpp.bioRxiv : the preprint server for biology · 2025Article
- The prodomain of bone morphogenetic protein 2 promotes dimerization and cleavage of BMP6 homodimers and BMP2/6 heterodimers.The Journal of biological chemistry · 2024Article
- Highly conserved and extremely evolvable: BMP signalling in secondary axis patterning of Cnidaria and Bilateria.Development genes and evolution · 2024Review
- Review
- Protection of the Prodomain α1-Helix Correlates with Latency in the Transforming Growth Factor-β Family.Journal of molecular biology · 2022Article
- Article
- Article
- TGF-β Family Signaling inCold Spring Harbor perspectives in biology · 2017Review
- Parallel Activin and BMP signaling coordinates R7/R8 photoreceptor subtype pairing in the stochasticeLife · 2017Article
- Tissue-specific regulation of BMP signaling byeLife · 2017Article
- The prodomain of BMP4 is necessary and sufficient to generate stable BMP4/7 heterodimers with enhanced bioactivity in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Simultaneous rather than ordered cleavage of two sites within the BMP4 prodomain leads to loss of ligand in mice.Development (Cambridge, England) · 2014Article
- Fine-tuned shuttles for bone morphogenetic proteins.Current opinion in genetics & development · 2013Review
- The factor VII-activating protease (FSAP) enhances the activity of bone morphogenetic protein-2 (BMP-2).The Journal of biological chemistry · 2013Article
- Multistep molecular mechanism for bone morphogenetic protein extracellular transport in the Drosophila embryo.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- A large bioactive BMP ligand with distinct signaling properties is produced by alternative proconvertase processing.Science signaling · 2012Article
- Regulation of BMP activity and range in Drosophila wing development.Current opinion in cell biology · 2012Review
- Different requirements for proteolytic processing of bone morphogenetic protein 5/6/7/8 ligands in Drosophila melanogaster.The Journal of biological chemistry · 2012Article
- Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
BMP4 is synthesized as an inactive precursor that is cleaved at two sites during maturation: initially at a site (S1) adjacent to the ligand domain, and then at an upstream site (S2) within the prodomain. Cleavage at the second site regulates the stability of mature BMP4 and this in turn influences its signaling intensity and range of action. The Drosophila ortholog of BMP4, Dpp, functions as a long- or short-range signaling molecule in the wing disc or embryonic midgut, respectively but mechanisms that differentially regulate its bioactivity in these tissues have not been explored. In the current studies we demonstrate, by dpp mutant rescue, that cleavage at the S2 site of proDpp is required for development of the wing and leg imaginal discs, whereas cleavage at the S1 site is sufficient to rescue Dpp function in the midgut. Both the S1 and S2 sites of proDpp are cleaved in the wing disc, and S2-cleavage is essential to generate sufficient ligand to exceed the threshold for pMAD activation at both short- and long-range in most cells. By contrast, proDpp is cleaved at the S1 site alone in the embryonic mesoderm and this generates sufficient ligand to activate physiological target genes in neighboring cells. These studies provide the first biochemical and genetic evidence that selective cleavage of the S2 site of proDPP provides a tissue-specific mechanism for regulating Dpp activity, and that differential cleavage can contribute to, but is not an absolute determinant of signaling range.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.