ArticleBMC biochemistry2010
Characterization of the first beta-class carbonic anhydrase from an arthropod (Drosophila melanogaster) and phylogenetic analysis of beta-class carbonic anhydrases in invertebrates.
Article in BMC biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 84 citations in OpenAlex.
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- Colchicine: A Dual Therapeutic Target for Trichinellosis.Acta parasitologica · 2025Article
- In silico evaluation of a new compound incorporating 4(3H)-quinazolinone and sulfonamide as a potential inhibitor of a human carbonic anhydrase.BMC chemistry · 2024Article
- Mechanistic characterization of a Drosophila model of paraneoplastic nephrotic syndrome.Nature communications · 2024Article
- Article
- Unique evolution of foraminiferal calcification to survive global changes.Science advances · 2023Article
- Potential Novel Role of Membrane-Associated Carbonic Anhydrases in the Kidney.International journal of molecular sciences · 2023Review
- Cloning, purification, kinetic and anion inhibition studies of a recombinant β-carbonic anhydrase from the Atlantic salmon parasite platyhelminthJournal of enzyme inhibition and medicinal chemistry · 2022Article
- A reverse vaccinology approach on transmembrane carbonic anhydrases from Plasmodium species as vaccine candidates for malaria prevention.Malaria journal · 2022Article
- Roles of Carbonic Anhydrases and Carbonic Anhydrase Related Proteins in Zebrafish.International journal of molecular sciences · 2022Review
- Assessment of databases to determine the validity of β- and γ-carbonic anhydrase sequences from vertebrates.BMC genomics · 2020Article
- The carbonic anhydrase of Clostridium autoethanogenum represents a new subclass of β-carbonic anhydrases.Applied microbiology and biotechnology · 2019Article
- Characterization, functional analysis, and expression levels of three carbonic anhydrases in response to pH and saline-alkaline stresses in the ridgetail white prawn Exopalaemon carinicauda.Cell stress & chaperones · 2019Article
- Anion inhibition studies of a beta carbonic anhydrase from the malaria mosquito Anopheles gambiae.Journal of enzyme inhibition and medicinal chemistry · 2018Article
- Involvement of β-Carbonic Anhydrase Genes in Bacterial Genomic Islands and Their Horizontal Transfer to Protists.Applied and environmental microbiology · 2018Article
- Carbonic anhydrase from Apis mellifera: purification and inhibition by pesticides.Journal of enzyme inhibition and medicinal chemistry · 2017Article
- Carbonic anhydrase enzyme as a potential therapeutic target for experimental trichinellosis.Parasitology research · 2016Article
- Horizontal transfer of β-carbonic anhydrase genes from prokaryotes to protozoans, insects, and nematodes.Parasites & vectors · 2016Article
- Innovative molecular diagnosis of Trichinella species based on β-carbonic anhydrase genomic sequence.Microbial biotechnology · 2016Article
- Carbonic Anhydrases in Cnidarians: Novel Perspectives from the Octocorallian Corallium rubrum.PloS one · 2016Article
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Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe beta-carbonic anhydrase (CA, EC 4.2.1.1) enzymes have been reported in a variety of organisms, but their existence in animals has been unclear. The purpose of the present study was to perform extensive sequence analysis to show that the beta-CAs are present in invertebrates and to clone and characterize a member of this enzyme family from a representative model organism of the animal kingdom, e.g., Drosophila melanogaster.
resultsThe novel beta-CA gene, here named DmBCA, was identified from FlyBase, and its orthologs were searched and reconstructed from sequence databases, confirming the presence of beta-CA sequences in 55 metazoan species. The corresponding recombinant enzyme was produced in Sf9 insect cells, purified, kinetically characterized, and its inhibition was investigated with a series of simple, inorganic anions. Holoenzyme molecular mass was defined by dynamic light scattering analysis and gel filtration, and the results suggested that the holoenzyme is a dimer. Double immunostaining confirmed predictions based on sequence analysis and localized DmBCA protein to mitochondria. The enzyme showed high CO2 hydratase activity, with a kcat of 9.5 x 105 s-1 and a kcat/KM of 1.1 x 108 M-1s-1. DmBCA was appreciably inhibited by the clinically-used sulfonamide acetazolamide, with an inhibition constant of 49 nM. It was moderately inhibited by halides, pseudohalides, hydrogen sulfide, bisulfite and sulfate (KI values of 0.67 - 1.36 mM) and more potently by sulfamide (KI of 0.15 mM). Bicarbonate, nitrate, nitrite and phenylarsonic/boronic acids were much weaker inhibitors (KIs of 26.9 - 43.7 mM).
conclusionsThe Drosophila beta-CA represents a highly active mitochondrial enzyme that is a potential model enzyme for anti-parasitic drug development.
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