ArticleMolecular microbiology2010
Removal of the outer Kdo from Helicobacter pylori lipopolysaccharide and its impact on the bacterial surface.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 56 citations in OpenAlex.
- Retargeting Gram-Positive-Only Adarotene-Derived Antibacterials to Broad-Spectrum Antibiotics.Antibiotics (Basel, Switzerland) · 2025Article
- Advances in Helicobacter pylori lipopolysaccharide structure and function.FEMS microbiology reviews · 2025Review
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- Single-step genome engineering in the bee gut symbiontbioRxiv : the preprint server for biology · 2023Article
- A Tripartite Efflux System Affects Flagellum Stability inInternational journal of molecular sciences · 2022Article
- Small RNA mediated gradual control of lipopolysaccharide biosynthesis affects antibiotic resistance in Helicobacter pylori.Nature communications · 2021Article
- Coarse-grained simulations uncover Gram-negative bacterial defense against polymyxins by the outer membrane.Computational and structural biotechnology journal · 2021Article
- HupA, the main undecaprenyl pyrophosphate and phosphatidylglycerol phosphate phosphatase in Helicobacter pylori is essential for colonization of the stomach.PLoS pathogens · 2019Article
- A Newly Discovered Drug Resistance GeneInfection and drug resistance · 2019Article
- Review
- Comparison of lipopolysaccharides composition of two different strains of Helicobacter pylori.BMC microbiology · 2017Article
- Helicobacter pylori modulates host cell responses by CagT4SS-dependent translocation of an intermediate metabolite of LPS inner core heptose biosynthesis.PLoS pathogens · 2017Article
- The redefinition of Helicobacter pylori lipopolysaccharide O-antigen and core-oligosaccharide domains.PLoS pathogens · 2017Article
- Lipopolysaccharide modification in Gram-negative bacteria during chronic infection.FEMS microbiology reviews · 2016Review
- Disruption of lipid homeostasis in the Gram-negative cell envelope activates a novel cell death pathway.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Current views of the relationship between Helicobacter pylori and Henoch-Schonlein purpura in children.World journal of clinical pediatrics · 2016Review
- Article
- Kdo2 -lipid A: structural diversity and impact on immunopharmacology.Biological reviews of the Cambridge Philosophical Society · 2015Review
- Helicobacter pylori: bacterial strategy for incipient stage and persistent colonization in human gastric niches.Yonsei medical journal · 2014Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Helicobacter pylori produces a unique surface lipopolysaccharide (LPS) characterized by strikingly low endotoxicity that is thought to aid the organism in evading the host immune response. This reduction in endotoxicity is predicted to arise from the modification of the Kdo-lipid A domain of Helicobacter LPS by a series of membrane bound enzymes including a Kdo (3-deoxy-d-manno-octulosonic acid) hydrolase responsible for the modification of the core oligosaccharide. Here, we report that Kdo hydrolase activity is dependent upon a putative two-protein complex composed of proteins Hp0579 and Hp0580. Inactivation of Kdo hydrolase activity produced two phenotypes associated with cationic antimicrobial peptide resistance and O-antigen expression. Kdo hydrolase mutants were highly sensitive to polymyxin B, which could be attributed to a defect in downstream modifications to the lipid A 4'-phosphate group. Production of a fully extended O-antigen was also diminished in a Kdo hydrolase mutant, with a consequent increase in core-lipid A. Finally, expression of O-antigen Lewis X and Y epitopes, known to mimic glycoconjugates found on human tissues, was also affected. Taken together, we have demonstrated that loss of Kdo hydrolase activity affects all three domains of H. pylori LPS, thus highlighting its role in the maintenance of the bacterial surface.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.