ArticleAssay and drug development technologies2010
Expanding the available assays: adapting and validating In-Cell Westerns in microfluidic devices for cell-based assays.
Article in Assay and drug development technologies, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 16 citations in OpenAlex.
- Heterotypic tumor spheroids: a platform for nanomedicine evaluation.Journal of nanobiotechnology · 2023Review
- The In-Cell Western immunofluorescence assay to monitor PROTAC mediated protein degradation.Methods in enzymology · 2023Article
- Characterization of the SIM-A9 cell line as a model of activated microglia in the context of neuropathic pain.PloS one · 2020Article
- Adaptation of a Simple Microfluidic Platform for High-Dimensional Quantitative Morphological Analysis of Human Mesenchymal Stromal Cells on Polystyrene-Based Substrates.SLAS technology · 2017Article
- Microphysiologic systems in female reproductive biology.Experimental biology and medicine (Maywood, N.J.) · 2017Review
- Single cell functional analysis of multiple myeloma cell populations correlates with diffusion profiles in static microfluidic coculture systems.Biomicrofluidics · 2016Article
- MicroC(3): an ex vivo microfluidic cis-coculture assay to test chemosensitivity and resistance of patient multiple myeloma cells.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Microengineered tumor models: insights & opportunities from a physical sciences-oncology perspective.Biomedical microdevices · 2013Review
- Real-time optical pH measurement in a standard microfluidic cell culture system.Biomedical optics express · 2013Article
- Opportunities and challenges for use of tumor spheroids as models to test drug delivery and efficacy.Journal of controlled release : official journal of the Controlled Release Society · 2012Review
- Microfluidic cell culture and its application in high-throughput drug screening: cardiotoxicity assay for hERG channels.Journal of biomolecular screening · 2011Article
- Automated high-throughput microchannel assays for cell biology: Operational optimization and characterization.JALA (Charlottesville, Va.) · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Microfluidic methods for cellular studies can significantly reduce costs due to reduced reagent and biological specimen requirements compared with many traditional culture techniques. However, current types of readouts are limited and this lack of suitable readouts for microfluidic cultures has significantly hindered the application of microfluidics for cell-based assays. The In-Cell Western (ICW) technique uses quantitative immunocytochemistry and a laser scanner to provide an in situ measure of protein quantities in cells grown in microfluidic channels of arbitrary geometries. The use of ICWs in microfluidic channels was validated by a detailed comparison with current macroscale methods and shown to have excellent correlation. Transforming growth factor-β-induced epithelial-to-mesenchymal transition of an epithelial cell line was used as an example for further validation of the technique as a readout for soluble-factor-based assays performed in high-throughput microfluidic channels. The use of passive pumping for sample delivery and laser scanning for analysis opens the door to high-throughput quantitative microfluidic cell-based assays that integrate seamlessly with existing high-throughput infrastructure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.