ArticlePloS one2010
Two origins for the gene encoding alpha-isopropylmalate synthase in fungi.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Response to leucine in Schizosaccharomyces pombe (fission yeast).FEMS yeast research · 2022Review
- Identification of QTL Associated with Regrowth Vigor Using the Nested Association Mapping Population in Switchgrass.Plants (Basel, Switzerland) · 2022Article
- Regulation of the Leucine Metabolism inJournal of fungi (Basel, Switzerland) · 2022Article
- Mystique ofJournal of genetics · 2018Article
- Genomic and Genetic Insights Into a Cosmopolitan Fungus,Frontiers in microbiology · 2018Article
- Article
- Reverse transcriptase and intron number evolution.Stem cell investigation · 2014Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe biosynthesis of leucine is a biochemical pathway common to prokaryotes, plants and fungi, but absent from humans and animals. The pathway is a proposed target for antimicrobial therapy. METHODOLOGY/PRINCIPAL
findingsHere we identified the leuA gene encoding alpha-isopropylmalate synthase in the zygomycete fungus Phycomyces blakesleeanus using a genetic mapping approach with crosses between wild type and leucine auxotrophic strains. To confirm the function of the gene, Phycomyces leuA was used to complement the auxotrophic phenotype exhibited by mutation of the leu3+ gene of the ascomycete fungus Schizosaccharomyces pombe. Phylogenetic analysis revealed that the leuA gene in Phycomyces, other zygomycetes, and the chytrids is more closely related to homologs in plants and photosynthetic bacteria than ascomycetes or basidiomycetes, and suggests that the Dikarya have acquired the gene more recently. CONCLUSIONS/SIGNIFICANCE: The identification of leuA in Phycomyces adds to the growing body of evidence that some primary metabolic pathways or parts of them have arisen multiple times during the evolution of fungi, probably through horizontal gene transfer events.
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