ArticleThe Journal of biological chemistry2010
Aquaporin tetramer composition modifies the function of tobacco aquaporins.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 159 citations in OpenAlex.
- A Ralstonia solanacearum effector RipAD perturbs aquaporin NbPIP oligomeric status to suppress plant immunity.The Plant journal : for cell and molecular biology · 2026Article
- Characterization and Computational Engineering of Structural Elements Controlling Gas Permeability in PIP2;1 Aquaporins.Journal of computational chemistry · 2026Article
- Amino Acid Substitutions in Loop C of Arabidopsis PIP2 Aquaporins Alters the Permeability of COPlant, cell & environment · 2025Article
- Analysis of sulfide signaling in rice highlights specific drought responses.Journal of experimental botany · 2024Article
- AtPIP1;4 and AtPIP2;4 Cooperatively Mediate HPlants (Basel, Switzerland) · 2024Article
- Aquaporins and COFrontiers in physiology · 2023Review
- A high-throughput yeast approach to characterize aquaporin permeabilities: Profiling the Arabidopsis PIP aquaporin sub-family.Frontiers in plant science · 2023Article
- Plasma membrane aquaporins of the PIP1 and PIP2 subfamilies facilitate hydrogen peroxide diffusion into plant roots.BMC plant biology · 2022Article
- Aquaporin Gating: A New Twist to Unravel Permeation through Water Channels.International journal of molecular sciences · 2022Review
- Mesophyll conductance is unaffected by expression of Arabidopsis PIP1 aquaporins in the plasmalemma of Nicotiana.Journal of experimental botany · 2022Article
- Expression of a COeLife · 2021Article
- Genome-Wide Identification, Structure Characterization, and Expression Pattern Profiling of the Aquaporin Gene Family inInternational journal of molecular sciences · 2021Article
- The contribution of PIP2-type aquaporins to photosynthetic response to increased vapour pressure deficit.Journal of experimental botany · 2021Article
- TheInternational journal of molecular sciences · 2021Article
- Genome-wide analysis of the aquaporin genes in melon (Cucumis melo L.).Scientific reports · 2020Article
- Controversial Regulation of Gene Expression and Protein Transduction of Aquaporins under Drought and Salinity Stress.Plants (Basel, Switzerland) · 2020Review
- A Survey of Barley PIP Aquaporin Ionic Conductance Reveals CaInternational journal of molecular sciences · 2020Article
- Evolutionary and Predictive Functional Insights into the Aquaporin Gene Family in the Allotetraploid PlantInternational journal of molecular sciences · 2020Article
- Sweet Cherry (International journal of molecular sciences · 2020Article
- Recent advances in understanding and improving photosynthesis.Faculty reviews · 2020Review
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterologous expression in yeast cells revealed that NtAQP1, a member of the so-called PIP1 aquaporin subfamily, did not display increased water transport activity in comparison with controls. Instead, an increased CO(2)-triggered intracellular acidification was observed. NtPIP2;1, which belongs to the PIP2 subfamily of plant aquaporins, behaved as a true aquaporin but lacked a CO(2)-related function. Results from split YFP experiments, protein chromatography, and gel electrophoresis indicated that the proteins form heterotetramers when coexpressed in yeast. Tetramer composition had effects on transport activity as demonstrated by analysis of artificial heterotetramers with a defined proportion of NtAQP1 to NtPIP2;1. A single NtPIP2;1 aquaporin in a tetramer was sufficient to significantly increase the water permeability of the respective yeast cells. With regard to CO(2)-triggered intracellular acidification, a cooperative effect was observed, where maximum rates were measured when the tetramer consisted of NtAQP1 aquaporins only. The results confirm the model of an aquaporin monomer as a functional unit for water transport and suggest that, for CO(2)-related transport processes, a structure built up by the tetramer is the basis of this function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.