ArticleApplied and environmental microbiology2010
Catabolite repression of Aox in Pichia pastoris is dependent on hexose transporter PpHxt1 and pexophagy.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 69 citations in OpenAlex.
- Yeast species resource exploration and application in cell factories.Synthetic and systems biotechnology · 2026Review
- Autophagic pathway is responsible for selective degradation of cytosolic enzymes formaldehyde and formate dehydrogenases in the methylotrophic yeast Komagataella phaffii.Scientific reports · 2026Article
- Systematic perturbation of cultures of K. phaffii by carbon co-feeding show gene signatures associated with production of recombinant proteins.Microbial cell factories · 2026Article
- Rewiring central metabolism in Komagataella phaffii for efficient mannose synthesis.Microbial cell factories · 2025Article
- Industrial Production of Proteins withBiomolecules · 2023Review
- Second generation Pichia pastoris strain and bioprocess designs.Biotechnology for biofuels and bioproducts · 2022Review
- Expanding the promoter toolbox for metabolic engineering of methylotrophic yeasts.Applied microbiology and biotechnology · 2022Review
- Advances in Cell Engineering of theMetabolites · 2022Review
- Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Transcriptome Analysis Unveils the Effects of Proline on Gene Expression in the YeastMicroorganisms · 2021Article
- Oxidative stress tolerance contributes to heterologous protein production in Pichia pastoris.Biotechnology for biofuels · 2021Article
- Yeast Expression Systems: Overview and Recent Advances.Molecular biotechnology · 2019Review
- Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1.BMC biotechnology · 2018Article
- Transcriptome analysis of Δmig1Δmig2 mutant reveals their roles in methanol catabolism, peroxisome biogenesis and autophagy in methylotrophic yeast Pichia pastoris.Genes & genomics · 2018Article
- Reduced methanol input induces increased protein output by AOX1 promoter in a trans-acting elements engineered Pichia pastoris.Journal of industrial microbiology & biotechnology · 2018Article
- Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris.Scientific reports · 2017Article
- Energy Charge as an Indicator of Pexophagy inFrontiers in microbiology · 2017Article
- A novel methanol-free Pichia pastoris system for recombinant protein expression.Microbial cell factories · 2016Article
- Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 (AOX1) Promoter in Pichia pastoris.The Journal of biological chemistry · 2016Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work, the identification and characterization of two hexose transporter homologs in the methylotrophic yeast Pichia pastoris, P. pastoris Hxt1 (PpHxt1) and PpHxt2, are described. When expressed in a Saccharomyces cerevisiae hxt-null mutant strain that is unable to take up monosaccharides, either protein restored growth on glucose or fructose. Both PpHXT genes are transcriptionally regulated by glucose. Transcript levels of PpHXT1 are induced by high levels of glucose, whereas transcript levels of PpHXT2 are relatively lower and are fully induced by low levels of glucose. In addition, PpHxt2 plays an important role in glycolysis-dependent fermentative growth, since PpHxt2 is essential for growth on glucose or fructose when respiration is inhibited. Notably, we firstly found that the deletion of PpHXT1, but not PpHXT2, leads to the induced expression of the alcohol oxidase I gene (AOX1) in response to glucose or fructose. We also elucidated that a sharp dropping of the sugar-induced expression level of Aox at a later growth phase is caused mainly by pexophagy, a degradation pathway in methylotrophic yeast. The sugar-inducible AOX1 promoter in an Deltahxt1 strain may be promising as a host for the expression of heterologous proteins. The functional analysis of these two hexose transporters is the first step in elucidating the mechanisms of sugar metabolism and catabolite repression in P. pastoris.
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