ArticleThe Journal of biological chemistry2010
Serine protease-mediated host invasion by the parasitic nematode Steinernema carpocapsae.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 54 citations in OpenAlex.
- In silico design of a T-cell epitope vaccine candidate for parasitic helminth infection.PLoS pathogens · 2020Pooled it
- Entomopathogenic NematodeToxins · 2026Article
- Applicability of Trichoderma spp. in sustainable agriculture against fungal and nematode phytopathogens: a review.Folia microbiologica · 2026Review
- Transcriptomic Analysis ofFrontiers in physiology · 2022Article
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- Genetic Diversity of Serine Protease Inhibitors in Myxozoan (Cnidaria, Myxozoa) Fish Parasites.Microorganisms · 2020Article
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- Participation of the Serine Protease Jonah66Ci in theInfection and immunity · 2019Article
- Editing the genome of Aphanomyces invadans using CRISPR/Cas9.Parasites & vectors · 2018Article
- Endosymbiont-based immunity in Drosophila melanogaster against parasitic nematode infection.PloS one · 2018Article
- Activated entomopathogenic nematode infective juveniles release lethal venom proteins.PLoS pathogens · 2017Article
- The genome, transcriptome, and proteome of the nematode Steinernema carpocapsae: evolutionary signatures of a pathogenic lifestyle.Scientific reports · 2016Article
- Genomics of Entomopathogenic Nematodes and Implications for Pest Control.Trends in parasitology · 2016Review
- Genome mining offers a new starting point for parasitology research.Parasitology research · 2015Review
- Serine proteases of parasitic helminths.The Korean journal of parasitology · 2015Review
- The genome of the myxosporean Thelohanellus kitauei shows adaptations to nutrient acquisition within its fish host.Genome biology and evolution · 2014Article
- Genome and transcriptome of the porcine whipworm Trichuris suis.Nature genetics · 2014Article
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- A serpin released by an entomopathogen impairs clot formation in insect defense system.PloS one · 2013Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Steinernema carpocapsae is an insect parasitic nematode used in biological control, which infects insects penetrating by mouth and anus and invading the hemocoelium through the midgut wall. Invasion has been described as a key factor in nematode virulence and suggested to be mediated by proteases. A serine protease cDNA from the parasitic stage was sequenced (sc-sp-1); the recombinant protein was produced in an Escherichia coli system, and a native protein was purified from the secreted products. Both proteins were confirmed by mass spectrometry to be encoded by the sc-sp-1 gene. Sc-SP-1 has a pI of 8.7, a molecular mass of 27.3 kDa, a catalytic efficiency of 22.2 × 10(4) s(-1) m(-1) against N-succinyl-Ala-Ala-Pro-Phe-pNA, and is inhibited by chymostatin (IC 0.07) and PMSF (IC 0.73). Sc-SP-1 belongs to the chymotrypsin family, based on sequence and biochemical analysis. Only the nematode parasitic stage expressed sc-sp-1. These nematodes in the midgut lumen, prepared to invade the insect hemocoelium, expressed higher levels than those already in the hemocoelium. Moreover, parasitic nematode sense insect peritrophic membrane and hemolymph more quickly than they do other tissues, which initiates sc-sp-1 expression. Ex vivo, Sc-SP-1 was able to bind to insect midgut epithelium and to cause cell detachment from basal lamina. In vitro, Sc-SP-1 formed holes in an artificial membrane model (Matrigel), whereas Sc-SP-1 treated with PMSF did not, very likely because it hydrolyzes matrix glycoproteins. These findings highlight the S. carpocapsae-invasive process that is a key step in the parasitism thus opening new perspectives for improving nematode virulence to use in biological control.
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Registered trials
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