ArticleBiochimica et biophysica acta2010
Neuron specific metabolic adaptations following multi-day exposures to oxygen glucose deprivation.
Article in Biochimica et biophysica acta, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
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- β-amyloid monomers drive up neuronal aerobic glycolysis in response to energy stressors.Aging · 2021Article
- Metabolic Reprogramming: Strategy for Ischemic Stroke Treatment by Ischemic Preconditioning.Biology · 2021Review
- Oscillations and concentration dynamics of brain tissue oxygen in neonates and adults.Journal of computational neuroscience · 2020Article
- Toxicity, recovery, and resilience in a 3D dopaminergic neuronal in vitro model exposed to rotenone.Archives of toxicology · 2018Article
- Neuronal Preconditioning Requires the Mitophagic Activity of C-terminus of HSC70-Interacting Protein.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Multianalyte Physiological Microanalytical Devices.Annual review of analytical chemistry (Palo Alto, Calif.) · 2017Review
- Monocarboxylate Transporter 1 in the Medial Prefrontal Cortex Developmentally Expresses in Oligodendrocytes and Associates with Neuronal Amounts.Molecular neurobiology · 2017Article
- Analysis of a Nitroreductase-Based Hypoxia Sensor in Primary Neuronal Cultures.ACS chemical neuroscience · 2016Article
- O-GlcNAcylation of ATG4B positively regulates autophagy by increasing its hydroxylase activity.Oncotarget · 2016Article
- CHIP Is an Essential Determinant of Neuronal Mitochondrial Stress Signaling.Antioxidants & redox signaling · 2015Article
- Extending injury- and disease-resistant CNS phenotypes by repetitive epigenetic conditioning.Frontiers in neurology · 2015Review
- Multichamber Multipotentiostat System for Cellular Microphysiometry.Sensors and actuators. B, Chemical · 2014Article
- The relevance and potential roles of microphysiological systems in biology and medicine.Experimental biology and medicine (Maywood, N.J.) · 2014Article
- Multianalyte microphysiometry reveals changes in cellular bioenergetics upon exposure to fluorescent dyes.Analytical chemistry · 2013Article
- Alteration of isocitrate dehydrogenase following acute ischemic injury as a means to improve cellular energetic status in neuroadaptation.CNS & neurological disorders drug targets · 2013Article
- Multianalyte Microphysiometry of Macrophage Responses to Phorbol Myristate Acetate, Lipopolysaccharide, and Lipoarabinomannan.Electroanalysis · 2013Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Prior exposure to sub toxic insults can induce a powerful endogenous neuroprotective program known as ischemic preconditioning. Current models typically rely on a single stress episode to induce neuroprotection whereas the clinical reality is that patients may experience multiple transient ischemic attacks (TIAs) prior to suffering a stroke. We sought to develop a neuron-enriched preconditioning model using multiple oxygen glucose deprivation (OGD) episodes to assess the endogenous protective mechanisms neurons implement at the metabolic and cellular level. We found that neurons exposed to a five minute period of glucose deprivation recovered oxygen utilization and lactate production using novel microphysiometry techniques. Using the non-toxic and energetically favorable five minute exposure, we developed a preconditioning paradigm where neurons are exposed to this brief OGD for three consecutive days. These cells experienced a 45% greater survival following an otherwise lethal event and exhibited a longer lasting window of protection in comparison to our previous in vitro preconditioning model using a single stress. As in other models, preconditioned cells exhibited mild caspase activation, an increase in oxidized proteins and a requirement for reactive oxygen species for neuroprotection. Heat shock protein 70 was upregulated during preconditioning, yet the majority of this protein was released extracellularly. We believe coupling this neuron-enriched multi-day model with microphysiometry will allow us to assess neuronal specific real-time metabolic adaptations necessary for preconditioning.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.