ArticleBiophysical journal2010
Refractive-index-based screening of membrane-protein-mediated transfer across biological membranes.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 36 citations in OpenAlex.
- Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipids and Lipid Membranes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Computing osmotic permeabilities of aquaporins AQP4, AQP5, and GlpF from near-equilibrium simulations.Biochimica et biophysica acta. Biomembranes · 2017Article
- 1,3-propanediol binds deep inside the channel to inhibit water permeation through aquaporins.Protein science : a publication of the Protein Society · 2016Article
- Optical waveguide lightmode spectroscopic techniques for investigating membrane-bound ion channel activities.PloS one · 2013Article
- Glycosylation increases the thermostability of human aquaporin 10 protein.The Journal of biological chemistry · 2011Article
- Controlling mass transport in microfluidic devices.Annual review of analytical chemistry (Palo Alto, Calif.) · 2011Review
- Sensing-applications of surface-based single vesicle arrays.Sensors (Basel, Switzerland) · 2010Review
- 1,3-Propanediol binds inside the water-conducting pore of aquaporin 4: Does this efficacious inhibitor have sufficient potency?Journal of systems and integrative neuroscienceArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Numerous membrane-transport proteins are major drug targets, and therefore a key ingredient in pharmaceutical development is the availability of reliable, efficient tools for membrane transport characterization and inhibition. Here, we present the use of evanescent-wave sensing for screening of membrane-protein-mediated transport across lipid bilayer membranes. This method is based on a direct recording of the temporal variations in the refractive index that occur upon a transfer-dependent change in the solute concentration inside liposomes associated to a surface plasmon resonance (SPR) active sensor surface. The applicability of the method is demonstrated by a functional study of the aquaglyceroporin PfAQP from the malaria parasite Plasmodium falciparum. Assays of the temperature dependence of facilitated diffusion of sugar alcohols on a single set of PfAQP-reconstituted liposomes reveal that the activation energies for facilitated diffusion of xylitol and sorbitol are the same as that previously measured for glycerol transport in the aquaglyceroporin of Escherichia coli (5 kcal/mole). These findings indicate that the aquaglyceroporin selectivity filter does not discriminate sugar alcohols based on their length, and that the extra energy cost of dehydration of larger sugar alcohols, upon entering the pore, is compensated for by additional hydrogen-bond interactions within the aquaglyceroporin pore.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.