ArticleBiochemical and biophysical research communications2010
Lipid raft facilitated ligation of K-alpha1-tubulin by specific antibodies on epithelial cells: Role in pathogenesis of chronic rejection following human lung transplantation.
Article in Biochemical and biophysical research communications, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
- Vascular restenosis in coronary artery bypass grafting might be associated with VEGF-C/VEGFR-3 signaling pathway.Heart and vessels · 2018Article
- Th17 Responses to Collagen Type V, kα1-Tubulin, and Vimentin Are Present Early in Human Development and Persist Throughout Life.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2017Article
- Immune Responses to Tissue-Restricted Nonmajor Histocompatibility Complex Antigens in Allograft Rejection.Journal of immunology research · 2017Review
- Sodium channel γENaC mediates IL-17 synergized high salt induced inflammatory stress in breast cancer cells.Cellular immunology · 2016Article
- Autoimmune Reactivity in Graft Injury: Player or Bystander?Current transplantation reports · 2015Article
- Protective role of bortezomib in steatotic liver ischemia/reperfusion injury through abrogation of MMP activation and YKL-40 expression.Transplant immunology · 2014Article
- Interplay between immune responses to HLA and non-HLA self-antigens in allograft rejection.Human immunology · 2013Review
- Pre-transplant antibodies to Kα1 tubulin and collagen-V in lung transplantation: clinical correlations.The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation · 2013Article
- Role of antibodies to self-antigens in chronic allograft rejection: potential mechanism and therapeutic implications.Human immunology · 2012Review
- Epithelial clara cell injury occurs in bronchiolitis obliterans syndrome after human lung transplantation.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2012Article
- T regulatory cells play a significant role in modulating MHC class I antibody-induced obliterative airway disease.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2012Article
- An important role for autoimmunity in the immunopathogenesis of chronic allograft rejection.International journal of immunogenetics · 2012Review
- HIF-1α signaling by airway epithelial cell K-α1-tubulin: role in fibrosis and chronic rejection of human lung allografts.Cellular immunology · 2012Article
- Immune responses to self-antigens (autoimmunity) in allograft rejection.Clinical transplants · 2012Article
- Chronic allograft dysfunction.Clinics in chest medicine · 2011Review
- A role for antibodies to human leukocyte antigens, collagen-V, and K-α1-Tubulin in antibody-mediated rejection and cardiac allograft vasculopathy.Transplantation · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Long term function of human lung allografts is hindered by development of chronic rejection manifested as Bronchiolitis Obliterans Syndrome (BOS). We have previously identified the development of antibodies (Abs) following lung transplantation to K-alpha1-tubulin (KAT), an epithelial surface gap junction cytoskeletal protein, in patients who develop BOS. However, the biochemical and molecular basis of the interactions and signaling cascades mediated by KAT Abs are yet to be defined. In this report, we investigated the biophysical basis of the epithelial cell membrane surface interaction between KAT and its specific Abs. Towards this, we analyzed the role of the lipid raft-domains in the membrane interactions which lead to cell signaling and ultimately increased growth factor expression. Normal human bronchial epithelial (NHBE) cells, upon specific ligation with Abs to KAT obtained either from the serum of BOS(+) patients or monoclonal KAT Abs, resulted in upregulation of growth factors VEGF, PDGF, and bFGF (6.4+/-1.1-, 3.2+/-0.9-, and 3.4+/-1.1-fold increase, respectively) all of which are important in the pathogenesis of BOS. To define the role for lipid raft in augmenting surface interactions, we analyzed the changes in the growth factor expression pattern upon depletion and enrichment with lipid raft following the ligation of the epithelial cell membranes with Abs specific for KAT. NHBE cells cultured in the presence of beta-methyl cyclodextran (betaMCD) had significantly reduced growth factor expression (1.3+/-0.3, vs betaMCD untreated being 6.4+/-1.1-fold increase) upon stimulation with KAT Abs. Depletion of cholesterol on NHBE cells upon treatment with betaMCD also resulted in decreased partitioning of caveolin in the membrane fraction indicating a decrease in raft-domains. In conclusion, our results demonstrate an important role for lipid raft-mediated ligation of Abs to KAT on the epithelial cell membrane, which results in the upregulation of growth factor cascades involved in the pathogenesis of BOS following human lung transplantation.
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