ArticleBMC microbiology2010
Development of stable reporter system cloning luxCDABE genes into chromosome of Salmonella enterica serotypes using Tn7 transposon.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- sRNA centered signaling activates nitrate respiration and enhances Cronobacter sakazakii virulence in host environments.Nature communications · 2026Article
- Engineered bacteria for near-infrared light-inducible expression of cancer therapeutics.Nature cancer · 2025Article
- Bioluminescent Pseudomonas aeruginosa and Escherichia coli for whole-cell screening of antibacterial and adjuvant compounds.Scientific reports · 2024Article
- Temperature dependence of the mutation rate towards antibiotic resistance.JAC-antimicrobial resistance · 2024Article
- Sonogenetics-controlled synthetic designer cells for cancer therapy in tumor mouse models.Cell reports. Medicine · 2024Article
- A Novel Antimicrobial Peptide Derived from Bony Fish IFN1 Exerts Potent Antimicrobial and Anti-Inflammatory Activity in Mammals.Microbiology spectrum · 2022Article
- A versatile TnPlant communications · 2022Article
- Preventing dysbiosis of the neonatal mouse intestinal microbiome protects against late-onset sepsis.Nature medicine · 2019Article
- Evaluation of in vitro and in vivo antibiotic efficacy against a novel bioluminescent Shigella flexneri.Scientific reports · 2019Article
- Article
- Sequestration from Protease Adaptor Confers Differential Stability to Protease Substrate.Molecular cell · 2017Article
- Salmonella enterica Serovar Kentucky Flagella Are Required for Broiler Skin Adhesion and Caco-2 Cell Invasion.Applied and environmental microbiology · 2017Article
- Small molecules targeting LapB protein prevent Listeria attachment to catfish muscle.PloS one · 2017Article
- A Modular, Tn7-Based System for Making Bioluminescent or Fluorescent Salmonella and Escherichia coli Strains.Applied and environmental microbiology · 2016Article
- Identification of Salmonella enterica serovar Kentucky genes involved in attachment to chicken skin.BMC microbiology · 2016Article
- Study on the promotion of bacterial biofilm formation by a Salmonella conjugative plasmid and the underlying mechanism.PloS one · 2014Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSalmonellosis may be a food safety problem when raw food products are mishandled and not fully cooked. In previous work, we developed bioluminescent Salmonella enterica serotypes using a plasmid-based reporting system that can be used for real-time monitoring of the pathogen's growth on food products in short term studies. In this study, we report the use of a Tn7-based transposon system for subcloning of luxCDABE genes into the chromosome of eleven Salmonella enterica serotypes isolated from the broiler production continuum.
resultsWe found that the lux operon is constitutively expressed from the chromosome post-transposition and the lux cassette is stable without external pressure, i.e. antibiotic selection, for all Salmonella enterica serotypes used. Bioluminescence expression is based on an active electron transport chain and is directly related with metabolic activity. This relationship was quantified by measuring bioluminescence against a temperature gradient in aqueous solution using a luminometer. In addition, bioluminescent monitoring of two serotypes confirmed that our chicken skin model has the potential to be used to evaluate pathogen mitigation strategies.
conclusionsThis study demonstrated that our new stable reporting system eliminates bioluminescence variation due to plasmid instability and provides a reliable real-time experimental system to study application of preventive measures for Salmonella on food products in real-time for both short and long term studies.
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