ArticleMobile DNA2010
Characterization of the TnsD-attTn7 complex that promotes site-specific insertion of Tn7.
Article in Mobile DNA, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- DNA remodeling couples target recognition to directional transposition in a Tn7-like CAST.bioRxiv : the preprint server for biology · 2026Article
- Programmable large-cargo integration: Overcoming size constraints for next-generation gene therapy.Synthetic and systems biotechnology · 2026Review
- Escherichia coli proteins uL29 and ACP stabilize the Tn7-encoded TnsD and its DNA binding.Mobile DNA · 2025Article
- Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons.Nucleic acids research · 2025Article
- A family of Tn7-like transposons evolved to target CRISPR repeats.Mobile DNA · 2025Article
- Characterization of halotolerant Kushneria isolates that stimulate growth of alfalfa in saline conditions.PloS one · 2025Article
- The genetic context of blaIMP varies among bacterial families from One Health sources.PloS one · 2025Article
- Article
- Assembly of the Tn7 targeting complex by a regulated stepwise process.Molecular cell · 2024Article
- Mobile-CRISPRi as a powerful tool for modulatingApplied and environmental microbiology · 2024Article
- Controlling the expression of heterologous genes in Bdellovibrio bacteriovorus using synthetic biology strategies.Microbial biotechnology · 2024Article
- Chromosomal barcodes for simultaneous tracking of near-isogenic bacterial strains in plant microbiota.Nature microbiology · 2024Article
- Article
- DNA on the move: mechanisms, functions and applications of transposable elements.FEBS open bio · 2024Review
- CRISPR-Associated Transposase for Targeted Mutagenesis in Diverse Proteobacteria.ACS synthetic biology · 2023Article
- Multiple adaptations underly co-option of a CRISPR surveillance complex for RNA-guided DNA transposition.Molecular cell · 2023Article
- Barcoding Populations of Pseudomonas fluorescens SBW25.Journal of molecular evolution · 2023Article
- Novel molecular requirements for CRISPR RNA-guided transposition.Nucleic acids research · 2023Article
- Insight into the molecular mechanism of the transposon-encoded type I-F CRISPR-Cas system.Journal, genetic engineering & biotechnology · 2023Review
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The bacterial transposon Tn7 is distinguished by its ability to recognize a specific site called attTn7, and insert just downstream of the highly conserved chromosomal glmS gene. TnsD is one of four transposon-encoded polypeptides (TnsABC+D) required for site-specific insertion of Tn7 into attTn7, and is the target site-selector that binds to a highly conserved sequence in the end of the glmS protein coding region. In this study, we identified important nucleotides within this region that are crucial for TnsD-attTn7 interaction. We also probed the regions of TnsD that interact with attTn7 and found that there are important DNA-binding determinants throughout the entire length of the protein, including an amino-terminal CCCH zinc-finger motif. A key role of TnsD is to recruit the non-sequence specific DNA-binding protein TnsC to attTn7; TnsC also interacts with and controls both the TnsA and TnsB subunits of the Tn7 transposase. TnsC stimulates the binding of TnsD to attTn7 in vivo, and TnsCD and TnsD can also interact in the absence of DNA and localize their interaction domains to the N-terminal region of each protein.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.