Evidence map›Paper›PMID 20653017›Full record

ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2010

Fluorogen-activating proteins as biosensors of cell-surface proteins in living cells.

John Holleran, Dara Brown, Margaret H Fuhrman, Sally A Adler, Gregory W Fisher, Jonathan W Jarvik

Abstract read
In one paragraph

Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Review
  6. Article
  7. Article
  8. Single-molecule labeling for studying trafficking of renal transporters.American journal of physiology. Renal physiology · 2018
    Review
  9. Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Fluorogenic Labeling Strategies for Biological Imaging.International journal of molecular sciences · 2017
    Review
  15. Article
  16. Article
  17. Article
  18. Flow Cytometry: Impact on Early Drug Discovery.Journal of biomolecular screening · 2015
    Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

John HolleranDepartment of Biological Sciences, Technology Center for Networks and Pathways, Molecular Biosensor and Imaging Center, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA.
Dara Brown
Margaret H Fuhrman
Sally A Adler
Gregory W Fisher
Jonathan W Jarvik

Funding

FLUORESCENT PROBES &IMAGING FOR NETWORKS &PATHWAY(RMI)U54RR022241 · NCRR · MELLON PITTS CORPORATION (MPC CORP) · PI WAGGONER, ALAN S. · 2005 to 2011
$19.9M
NCRR NIH HHS U54 RR02NCRR NIH HHS U54 RR022241
6 · The paper itself

Abstract

This study explores the general utility of a new class of biosensor that allows one to selectively visualize molecules of a chosen membrane protein that are at the cell surface. These biosensors make use of recently described bipartite fluoromodules comprised of a fluorogen-activating protein (FAP) and a small molecule (fluorogen) whose fluorescence increases dramatically when noncovalently bound by the FAP (Szent-Gyorgyi et al., Nat Biotechnol 2010;00:000-000).

Indexed as

Adrenergic beta-2 Receptor AgonistsAnimalsBiosensing TechniquesCell MembraneCell SurvivalCystic Fibrosis Transmembrane Conductance RegulatorEndocytosisFluorescent DyesGlucose Transporter Type 4HumansMembrane ProteinsMiceMicroscopy, FluorescenceNIH 3T3 CellsProtein Structure, TertiaryReceptors, Adrenergic, beta-2Adrenergic beta-2 Receptor AgonistsCFTR protein, humanCystic Fibrosis Transmembrane Conductance RegulatorFluorescent DyesGlucose Transporter Type 4Membrane ProteinsReceptors, Adrenergic, beta-2SLC2A4 protein, human

Identifiers

PMID20653017
PMCPMC2945705

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.