ArticleWorld journal of surgery2010
Detection of papillary thyroid carcinoma by analysis of BRAF and RET/PTC1 mutations in fine-needle aspiration biopsies of thyroid nodules.
Article in World journal of surgery, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 71 citations in OpenAlex.
- Real-world EUROCRINEInnovative surgical sciences · 2022Article
- Follicular Growth Pattern Disease on Thyroid Fine-needle Aspiration BiopsyBalkan medical journal · 2022Article
- Prevalence ofGland surgery · 2020Review
- Pitfalls in Thyroid Cytopathology.Surgical pathology clinics · 2019Review
- Review
- Molecular testing in the diagnosis of differentiated thyroid carcinomas.Gland surgery · 2018Review
- [Surgery of papillary thyroid microcarcinoma].Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen · 2018Review
- Rapid methods to create a positive control and identify theOncotarget · 2018Article
- Surgical Treatment in Papillary Thyroid Microcarcinoma.Sisli Etfal Hastanesi tip bulteni · 2018Review
- Multiplex PCR approach to simultaneously identify several mutations in fine needle cytology thyroid samples.Oncotarget · 2017Article
- Diagnostic Limitation of Fine-Needle Aspiration (FNA) on Indeterminate Thyroid Nodules Can Be Partially Overcome by Preoperative Molecular Analysis: Assessment of RET/PTC1 Rearrangement in BRAF and RAS Wild-Type Routine Air-Dried FNA Specimens.International journal of molecular sciences · 2017Article
- Non-invasive diagnosis of papillary thyroid microcarcinoma: a NMR-based metabolomics approach.Oncotarget · 2016Article
- Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules.Endocrinology and metabolism (Seoul, Korea) · 2016Article
- Diagnostic value of BRAF (V600E)-mutation analysis in fine-needle aspiration of thyroid nodules: a meta-analysis.OncoTargets and therapy · 2016Article
- [Clinical importance of thyroid gland cytology].Der Pathologe · 2015Review
- Mutation Profile of Well-Differentiated Thyroid Cancer in Asians.Endocrinology and metabolism (Seoul, Korea) · 2015Review
- Thyroglossal duct cyst cancer most likely arises from a thyroid gland remnant.Virchows Archiv : an international journal of pathology · 2014Article
- Molecular diagnostics of fine needle aspiration for the presurgical screening of thyroid nodules.Current genomics · 2014Article
- Update on the molecular diagnosis and targeted therapy of thyroid cancer.Medical oncology (Northwood, London, England) · 2014Review
- Contribution of the BRAF oncogene in the pre-operative phase of thyroid carcinoma.Oncology letters · 2013Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundActivating mutations of the oncogene BRAF or rearrangements of the tyrosine kinase receptor RET are observed in up to 80% of papillary thyroid carcinomas (PTCs). The predominant BRAF V600E mutation has not been detected in benign thyroid tissue so far, so consequently, this assumedly pathognomonic alteration is qualified to improve the preoperative diagnosis of PTC.
methodsTwo hundred ninety preoperatively harvested fine-needle aspiration biopsies (FNABs) underwent routine cytologic assessment. BRAF V600E mutation analysis was performed by mutation-specific PCR using the same cell material; a hybrid-specific RT-PCR assay was used for detection of RET/PTC1 rearrangements. Detected genetic alterations were verified by direct sequencing. Definitive histopathology was obtained in 93/290 lesions following surgery of the respective thyroid nodule.
resultsWhile cytology alone diagnosed 13/30 malignancies (22 PTCs, 4 FTCs, 1 MTC, 1 UTC, 2 metastases), five additional malignancies were identified by supplementary mutation analysis. Cytology classified eight FNABs as benign, while postoperative histology demonstrated a thyroid malignancy (6 PTCs, 1 FTC, 1 metastasis). In four of these eight cases, the genetic analysis detected a BRAF V600E mutation or a RET/PTC1 rearrangement. Classifying both suspicious and malignant FNAB results as positive cytology results, supplementary genetic testing increased the overall sensitivity of FNAB from 70.4 to 85.7%, the positive predictive value (PPV) from 59.4 to 64.9%, and the negative predictive value (NPV) from 84.0 to 91.3%.
conclusionsSupplementary mutation analysis of RET and especially of the BRAF V600E mutation in FNABs is a fast and probably cost-effective assay in routine diagnostic setting. Mutation analyses of PTC-specific genetic alterations improve the preoperative identification and prognostic assessment of thyroid malignancies and therefore enable an optimized surgical strategy.
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