ArticleInternational journal of hydrogen energy2010
Elimination of Rubisco alters the regulation of nitrogenase activity and increases hydrogen production in Rhodospirillum rubrum.
Article in International journal of hydrogen energy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 76 citations in OpenAlex.
- Nitrogenase regulation inbioRxiv : the preprint server for biology · 2026Article
- Redox poise in R. rubrum phototrophic growth drives large-scale changes in macromolecular pathways.PLoS computational biology · 2025Article
- Quantitative Modelling of Biohydrogen Production from Indian Agricultural Residues via Dark Fermentation.ChemistryOpen · 2025Article
- Solar-Driven Hydrogen Peroxide Production via BiVOAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Metabolic pathways to sustainability: review of purple non-sulfur bacteria potential in agri-food waste valorization.Frontiers in bioengineering and biotechnology · 2025Review
- High-Valence Oxides for High Performance Oxygen Evolution Electrocatalysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Nitrogen Fixation and Ammonium Assimilation Pathway Expression of Geobacter sulfurreducens Changes in Response to the Anode Potential in Microbial Electrochemical Cells.Applied and environmental microbiology · 2023Article
- Superior hydrogen performance ofRSC advances · 2023Article
- Unlocking the potential of polymeric desalination membranes by understanding molecular-level interactions and transport mechanisms.Chemical science · 2023Review
- Plasma-Assisted Reforming of Methane.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- The Conversion of Carbon Monoxide and Carbon Dioxide by Nitrogenases.Chembiochem : a European journal of chemical biology · 2022Review
- A directed genome evolution method to enhance hydrogen production inFrontiers in microbiology · 2022Article
- Effects of Mixing Volatile Fatty Acids as Carbon Sources onMicroorganisms · 2021Article
- TCA Cycle Replenishing Pathways in Photosynthetic Purple Non-Sulfur Bacteria Growing with Acetate.Life (Basel, Switzerland) · 2021Review
- Estimation of total energy requirement for sewage treatment by a microbial fuel cell with a one-meter air-cathode assuming Michaelis-Menten COD degradation.RSC advances · 2021Article
- Improving the dynamics of a Nd-Mg-Ni-based alloy by combining Ni element and mechanical milling.RSC advances · 2021Article
- Analysis of the Involvement of the Isoleucine Biosynthesis Pathway in Photoheterotrophic Metabolism ofFrontiers in microbiology · 2021Article
- Simultaneous wastewater treatment and energy harvesting in microbial fuel cells: an update on the biocatalysts.RSC advances · 2020Review
- Urine in Bioelectrochemical Systems: An Overall Review.ChemElectroChem · 2020Review
- Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Nitrogenase not only reduces atmospheric nitrogen to ammonia, but also reduces protons to hydrogen (H(2)). The nitrogenase system is the primary means of H(2) production under photosynthetic and nitrogen-limiting conditions in many photosynthetic bacteria, including Rhodospirillum rubrum. The efficiency of this biological H(2) production largely depends on the nitrogenase enzyme and the availability of ATP and electrons in the cell. Previous studies showed that blockage of the CO(2) fixation pathway in R. rubrum induced nitrogenase activity even in the presence of ammonium, presumably to remove excess reductant in the cell. We report here the re-characterization of cbbM mutants in R. rubrum to study the effect of Rubisco on H(2) production. Our newly constructed cbbM mutants grew poorly in malate medium under anaerobic conditions. However, the introduction of constitutively active NifA (NifA*), the transcriptional activator of the nitrogen fixation (nif) genes, allows cbbM mutants to dissipate the excess reductant through the nitrogenase system and improves their growth. Interestingly, we found that the deletion of cbbM alters the posttranslational regulation of nitrogenase activity, resulting in partially active nitrogenase in the presence of ammonium. The combination of mutations in nifA, draT and cbbM greatly increased H(2) production of R. rubrum, especially in the presence of excess of ammonium. Furthermore, these mutants are able to produce H(2) over a much longer time frame than the wild type, increasing the potential of these recombinant strains for the biological production of H(2).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.