ArticlePLoS neglected tropical diseases2010
The Jacob2 lectin of the Entamoeba histolytica cyst wall binds chitin and is polymorphic.
Article in PLoS neglected tropical diseases, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
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- Morphological and Motility Features of the Stable Bleb-Driven Monopodial Form ofInfection and immunity · 2020Article
- The most abundant cyst wall proteins of Acanthamoeba castellanii are lectins that bind cellulose and localize to distinct structures in developing and mature cyst walls.PLoS neglected tropical diseases · 2019Article
- Flow cytometric characterization of encystation in Entamoeba invadens.Molecular and biochemical parasitology · 2017Article
- Species-Specific Immunodetection of an Entamoeba histolytica Cyst Wall Protein.PLoS neglected tropical diseases · 2016Article
- Proteomic Study of Entamoeba histolytica Trophozoites, Cysts, and Cyst-Like Structures.PloS one · 2016Article
- Strategies to discover the structural components of cyst and oocyst walls.Eukaryotic cell · 2013Review
- Proteomic analysis of the cyst stage of Entamoeba histolytica.PLoS neglected tropical diseases · 2012Article
- Toward low-cost affinity reagents: lyophilized yeast-scFv probes specific for pathogen antigens.PloS one · 2012Article
- A simple fibril and lectin model for cyst walls of Entamoeba and perhaps Giardia.Trends in parasitology · 2011Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundThe infectious and diagnostic form of Entamoeba histolytica (Eh), cause of amebic dysentery and liver abscess, is the quadranucleate cyst. The cyst wall of Entamoeba invadens (Ei), a model for Eh, is composed of chitin fibrils and three sets of chitin-binding lectins that cross-link chitin fibrils (multivalent Jacob lectins), self-aggregate (Jessie lectins), and remodel chitin (chitinase). The goal here was to determine how well the Ei model applies to Entamoeba cysts from humans. METHODS/
resultsAn Eh Jacob lectin (EhJacob2) has three predicted chitin-binding domains surrounding a large, Ser-rich spacer. Recombinant EhJacob2 made in transfected Eh trophozoites binds to particulate chitin. Sequences of PCR products using primers flanking the highly polymorphic spacer of EhJacob2 may be used to distinguish Entamoeba isolates. Antibodies to the EhJacob2, EhJessie3, and chitinase each recognize cyst walls of clinical isolates of Entamoeba. While numerous sera from patients with amebic intestinal infections and liver abscess recognize recombinant EhJacob1 and EhJessie3 lectins, few of these sera recognize recombinant EhJacob2. CONCLUSIONS/SIGNIFICANCE: The EhJacob2 lectin binds chitin and is polymorphic, and Jacob2, Jessie3, and chitinase are present in cyst walls of clinical isolates of Entamoeba. These results suggest there are substantial similarities between cysts of the human pathogen (Eh) and the in vitro model (Ei), even though there are quantitative and qualitative differences in their chitin-binding lectins.
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