ArticleHuman molecular genetics2010
Centromere protein A dynamics in human pluripotent stem cell self-renewal, differentiation and DNA damage.
Article in Human molecular genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- Fully T2T pedigree assemblies reveal genetic stability and epigenetic plasticity of human centromeres across inheritance and cell-fate transitions.bioRxiv : the preprint server for biology · 2026Article
- Mitotic drive in asymmetric epigenetic inheritance.Biochemical Society transactions · 2022Article
- Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- CENP-A: A Histone H3 Variant with Key Roles in Centromere Architecture in Healthy and Diseased States.Results and problems in cell differentiation · 2022Article
- LncRNA MBNL1-AS1 Represses Proliferation and Cancer Stem-Like Properties of Breast Cancer through MBNL1-AS1/ZFP36/CENPA Axis.Journal of oncology · 2022Article
- CENPA promotes clear cell renal cell carcinoma progression and metastasis via Wnt/β-catenin signaling pathway.Journal of translational medicine · 2021Article
- CENP-C functions in centromere assembly, the maintenance of CENP-A asymmetry and epigenetic age in Drosophila germline stem cells.PLoS genetics · 2021Article
- CENP-A overexpression promotes distinct fates in human cells, depending on p53 status.Communications biology · 2021Article
- Chromatin Regulation in Development: Current Understanding and Approaches.Stem cells international · 2021Review
- DNA Double-Strand Break Repair: All Roads Lead to HeterochROMAtin Marks.Frontiers in genetics · 2021Review
- Article
- Genetic and epigenetic effects on centromere establishment.Chromosoma · 2020Review
- DNA damage response and spindle assembly checkpoint function throughout the cell cycle to ensure genomic integrity.PLoS genetics · 2015Article
- CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells.Epigenetics & chromatin · 2015Article
- PRMT5 is required for human embryonic stem cell proliferation but not pluripotency.Stem cell reviews and reports · 2014Article
- CENP-A is essential for cardiac progenitor cell proliferation.Cell cycle (Georgetown, Tex.) · 2014Article
- Stage-specific roles for tet1 and tet2 in DNA demethylation in primordial germ cells.Cell stem cell · 2013Article
- Centromere protein-A, an essential centromere protein, is a prognostic marker for relapse in estrogen receptor-positive breast cancer.Breast cancer research : BCR · 2012Article
- Flexibility of centromere and kinetochore structures.Trends in genetics : TIG · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Human pluripotent stem cells (hPSCs) hold significant promise for use in regenerative medicine, or as a model to understand human embryo development. However, the basic mechanisms required for proliferation and self-renewal of hPSCs have not been fully uncovered. Proliferation in all eukaryotes is dependent upon highly regulated expression of the histone H3 variant Centromere protein A (CENP-A). In the current study, we demonstrate that hPSCs have a unique messenger ribonucleic acid (mRNA) reserve of CENP-A not found in somatic fibroblasts. Using short hairpin RNA technology to reduce but not ablate CENP-A, we show that CENP-A-depleted hPSCs are still capable of maintaining a functional centromeric mark, whereas fibroblasts are not. However, upon induction of differentiation or DNA damage, hPSCs with depleted CENP-A arrest in G2/M and undergo apoptosis. Analysis of CENP-A dynamics following DNA damage in hPSCs reveals that 60 min after irradiation, CENP-A is found in multiple small nuclear foci that are mutually exclusive to γH2AX as well as CENP-C. Furthermore, following irradiation, hPSCs with depleted CENP-A mount a normal apoptotic response at 6 h; however at 24 h, apoptosis is significantly increased in CENP-A-depleted hPSCs relative to control. Taken together, our results indicate that hPSCs exhibit a unique mechanism for maintaining genomic integrity by possessing the flexibility to reduce the amount of CENP-A required to maintain a functional centromere under self-renewing conditions, and maintaining a reserve of CENP-A mRNA to rebuild the centromere following differentiation or DNA damage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.