ArticleThe Journal of biological chemistry2010
Identification of phosphomethylethanolamine N-methyltransferase from Arabidopsis and its role in choline and phospholipid metabolism.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 37 citations in OpenAlex.
- Remodeling of leaf lipidome during cold acclimation, de-acclimation, and re-acclimation influences frost tolerance of Festuca arundinacea.Plant & cell physiology · 2026Article
- A Comprehensive Functional Analysis ofPlants (Basel, Switzerland) · 2025Article
- NAC32 alleviates magnesium toxicity-induced cell death through positive regulation of XIPOTL1 expression.Plant physiology · 2023Article
- Cloning and Functional Identification of Phosphoethanolamine Methyltransferase in Soybean (Frontiers in plant science · 2021Article
- A Methyltransferase Trio Essential for Phosphatidylcholine Biosynthesis and Growth.Plant physiology · 2019Article
- Loss of PhosphoethanolaminePlant physiology · 2019Article
- NMT1 and NMT3Plant physiology · 2018Article
- Pi starvation-dependent regulation of ethanolamine metabolism by phosphoethanolamine phosphatase PECP1 in Arabidopsis roots.Journal of experimental botany · 2018Article
- Cloning and Functional Analysis of Phosphoethanolamine Methyltransferase Promoter from Maize (Zea mays L.).International journal of molecular sciences · 2018Article
- Covering their bases: The phosphobase methylation pathway in plants.The Journal of biological chemistry · 2017Article
- Conformational changes in the di-domain structure ofThe Journal of biological chemistry · 2017Article
- Glycerolipid synthesis and lipid trafficking in plant mitochondria.The FEBS journal · 2017Review
- C1 Metabolism Inhibition and Nitrogen Deprivation Trigger Triacylglycerol Accumulation inFrontiers in plant science · 2016Article
- PHOSPHATIDIC ACID PHOSPHOHYDROLASE Regulates Phosphatidylcholine Biosynthesis in Arabidopsis by Phosphatidic Acid-Mediated Activation of CTP:PHOSPHOCHOLINE CYTIDYLYLTRANSFERASE Activity.The Plant cell · 2015Article
- Lipid biosynthesis and protein concentration respond uniquely to phosphate supply during leaf development in highly phosphorus-efficient Hakea prostrata.Plant physiology · 2014Article
- Molecular mapping of Arabidopsis thaliana lipid-related orthologous genes in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
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5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three sequential methylations of phosphoethanolamine (PEA) are required for the synthesis of phosphocholine (PCho) in plants. A cDNA encoding an N-methyltransferase that catalyzes the last two methylation steps was cloned from Arabidopsis by heterologous complementation of a Saccharomyces cerevisiae cho2, opi3 mutant. The cDNA encodes phosphomethylethanolamine N-methyltransferase (PMEAMT), a polypeptide of 475 amino acids that is organized as two tandem methyltransferase domains. PMEAMT shows 87% amino acid identity to a related enzyme, phosphoethanolamine N-methyltransferase, an enzyme in plants that catalyzes all three methylations of PEA to PCho. PMEAMT cannot use PEA as a substrate, but assays using phosphomethylethanolamine as a substrate result in both phosphodimethylethanolamine and PCho as products. PMEAMT is inhibited by the reaction products PCho and S-adenosyl-l-homocysteine, a property reported for phosphoethanolamine N-methyltransferase from various plants. An Arabidopsis mutant with a T-DNA insertion associated with locus At1g48600 showed no transcripts encoding PMEAMT. Shotgun lipidomic analyses of leaves of atpmeamt and wild-type plants generated phospholipid profiles showing the content of phosphatidylmethylethanolamine to be altered relative to wild type with the content of a 34:3 lipid molecular species 2-fold higher in mutant plants. In S. cerevisiae, an increase in PtdMEA in membranes is associated with reduced viability. This raises a question regarding the role of PMEAMT in plants and whether it serves to prevent the accumulation of PtdMEA to potentially deleterious levels.
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