ArticleBiology of reproduction2010
Retinoic acid availability drives the asynchronous initiation of spermatogonial differentiation in the mouse.
Article in Biology of reproduction, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
54 citing papers in PubMed, 111 citations in OpenAlex.
- Azoospermia phenotype and scRNA-seq reveal hnRNPK as a factor essential for male germ cell development in mice.Nucleic acids research · 2026Article
- Testicular Histological Changes and Ki-67 Expression in Lead (II) Oxide Toxicity among Neonatal Wistar Rats and Modulatory Role of Retinol.Archives of Razi Institute · 2025Article
- 3D Bioprinted Coaxial Testis Model Using Human Induced Pluripotent Stem Cells:A Step Toward Bicompartmental Cytoarchitecture and Functionalization.Advanced healthcare materials · 2025Article
- Enrichment of spermatogonial stem cells and staging of the testis cycle in a dasyurid marsupial, the fat-tailed dunnart.Stem cells (Dayton, Ohio) · 2025Article
- Review
- Isolation of Homogeneous Sub-populations of Spermatocytes from Mouse Testis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Core binding factor subunit β plays diverse and essential roles in the male germline.Frontiers in cell and developmental biology · 2023Article
- Identification and Expression Pattern ofAnimals : an open access journal from MDPI · 2022Article
- Article
- Unique metabolic phenotype and its transition during maturation of juvenile male germ cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- FSH regulates RA signaling to commit spermatogonia into differentiation pathway and meiosis.Reproductive biology and endocrinology : RB&E · 2021Review
- The rapamycin analog Everolimus reversibly impairs male germ cell differentiation and fertility in the mouse†.Biology of reproduction · 2020Article
- ADAD1 and ADAD2, testis-specific adenosine deaminase domain-containing proteins, are required for male fertility.Scientific reports · 2020Article
- Review
- Retinoic Acid and Germ Cell Development in the Ovary and Testis.Biomolecules · 2019Review
- Retinoic acid signaling pathways.Development (Cambridge, England) · 2019Review
- Retinoic acid signaling and the cycle of the seminiferous epithelium.Current opinion in endocrine and metabolic research · 2019Article
- Differential RA responsiveness directs formation of functionally distinct spermatogonial populations at the initiation of spermatogenesis in the mouse.Development (Cambridge, England) · 2019Article
- Retinoic acid receptor signaling is necessary in steroidogenic cells for normal spermatogenesis and epididymal function.Development (Cambridge, England) · 2018Article
- 50 years of spermatogenesis: Sertoli cells and their interactions with germ cells.Biology of reproduction · 2018Review
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3 authors at 1 institution in 1 country.
Funding
Abstract
Throughout the reproductive lifespan of most male mammals, sperm production is constant because of the regulated differentiation of spermatogonia. Retinoic acid (RA) and a downstream target, Stra8, are required for complete spermatogenesis. To examine the role of RA in initiating spermatogonial differentiation, a transgenic mouse model expressing beta-galactosidase under the control of an RA response element was used. Cells in the neonatal testis undergoing active RA signaling were visualized by beta-galactosidase activity, the relationship between RA and differentiation determined, and the role of RA-degrading enzymes in regulating RA demonstrated. Beta-galactosidase activity was found to be predominantly associated with differentiating, premeiotic germ cells and to be distributed nonuniformly throughout the seminiferous tubules. Additionally, beta-galactosidase activity in premeiotic germ cells colocalized with STRA8 protein and was induced in germ cells with exogenous RA treatment. The RA-degrading enzyme, CYP26B1, was found to have germ cell localization and nonuniform distribution between tubules via immunohistochemistry. Treatment with a CYP26 enzyme inhibitor resulted in an increased number of germ cells with both beta-galactosidase activity and STRA8 protein and an increase in the expression of genes associated with differentiation and reduced expression of a gene associated with undifferentiated germ cells. These results show the action of RA in a subset of spermatogonia leads to nonuniform initiation of differentiation throughout the neonatal testis, potentially mediated through the action of CYP26 enzymes. Thus, the presence of RA is a likely driving factor in the initiation of spermatogonial differentiation and may result in asynchronous spermatogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.