ArticleMolecular and cellular biology2010
Targeted 2'-O methylation at a nucleotide within the pseudoknot of telomerase RNA reduces telomerase activity in vivo.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Site-specific inhibition of translation initiation via 2'-O-methylation.Nucleic acids research · 2026Article
- OMetaNet: an efficient hybrid deep learning model based on multimodal data fusion and contrastive learning for predicting 2'-O-methylation sites in human RNA.BMC bioinformatics · 2025Article
- snoRNA-facilitated protein secretion revealed by transcriptome-wide snoRNA target identification.Cell · 2025Article
- H2Opred: a robust and efficient hybrid deep learning model for predicting 2'-O-methylation sites in human RNA.Briefings in bioinformatics · 2023Article
- From Antisense RNA to RNA Modification: Therapeutic Potential of RNA-Based Technologies.Biomedicines · 2021Review
- Detection and quantification of RNA 2'-O-methylation and pseudouridylation.Methods (San Diego, Calif.) · 2016Article
- Article
- Synthesis and labeling of RNA in vitro.Current protocols in molecular biology · 2013Article
- Characterization of an HIV-targeted transcriptional gene-silencing RNA in primary cells.Human gene therapy · 2012Article
- Thermodynamic characterization of the Saccharomyces cerevisiae telomerase RNA pseudoknot domain in vitro.RNA (New York, N.Y.) · 2012Article
- Inducing nonsense suppression by targeted pseudouridylation.Nature protocols · 2012Article
- The emerging role of triple helices in RNA biology.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Telomerase RNA is an essential component of telomerase, a ribonucleoprotein enzyme that maintains chromosome ends in most eukaryotes. Here we employ a novel approach, namely, RNA-guided RNA modification, to assess whether introducing 2'-O methylation into telomerase RNA can influence telomerase activity in vivo. We generate specific 2'-O methylation sites in and adjacent to the triple helix (within the conserved pseudoknot structure) of Saccharomyces cerevisiae telomerase RNA (TLC1). We show that 2'-O methylation at U809 reduces telomerase activity, resulting in telomere shortening, whereas 2'-O methylation at A804 or A805 leads to moderate telomere lengthening. Importantly, we also show that targeted 2'-O methylation does not affect TLC1 levels and that 2'-O-methylated TLC1 appears to be efficiently assembled into telomerase ribonucleoprotein. Our results demonstrate that RNA-guided RNA modification is a highly useful approach for modulating telomerase activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.