ArticleMolecular and cellular biology2010
Single-molecule analysis reveals changes in the DNA replication program for the POU5F1 locus upon human embryonic stem cell differentiation.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Impact of Tau overexpression on DNA replication dynamics in centromeres of human neural progenitor cells.iScience · 2026Article
- DNA polymerase zeta contributes to heterochromatin replication to prevent genome instability.The EMBO journal · 2021Article
- Pluripotent stem cells with low differentiation potential contain incompletely reprogrammed DNA replication.The Journal of cell biology · 2020Article
- The Replication of Frataxin Gene Is Assured by Activation of Dormant Origins in the Presence of a GAA-Repeat Expansion.PLoS genetics · 2016Article
- Replication timing and transcriptional control: beyond cause and effect-part III.Current opinion in cell biology · 2016Review
- Dynamic changes in replication timing and gene expression during lineage specification of human pluripotent stem cells.Genome research · 2015Article
- Peaks cloaked in the mist: the landscape of mammalian replication origins.The Journal of cell biology · 2015Review
- Mapping of Replication Origins in the X Inactivation Center of Vole Microtus levis Reveals Extended Replication Initiation Zone.PloS one · 2015Article
- Epigenetic landscape for initiation of DNA replication.Chromosoma · 2014Review
- DNA replication timing.Cold Spring Harbor perspectives in biology · 2013Review
- Genome-wide copy number profiling of single cells in S-phase reveals DNA-replication domains.Nucleic acids research · 2013Article
- Global analysis reveals multiple pathways for unique regulation of mRNA decay in induced pluripotent stem cells.Genome research · 2012Article
- Human telomeres replicate using chromosome-specific, rather than universal, replication programs.The Journal of cell biology · 2012Article
- Regulation of DNA replication during development.Development (Cambridge, England) · 2012Review
- DNA replication fading as proliferating cells advance in their commitment to terminal differentiation.Scientific reports · 2012Article
- Regulation of DNA replication timing on human chromosome by a cell-type specific DNA binding protein SATB1.PloS one · 2012Article
- Evidence for sequential and increasing activation of replication origins along replication timing gradients in the human genome.PLoS computational biology · 2011Article
- Single molecule analysis of replicated DNA reveals the usage of multiple KSHV genome regions for latent replication.PLoS pathogens · 2011Article
- Genomic approaches to the initiation of DNA replication and chromatin structure reveal a complex relationship.Briefings in functional genomics · 2011Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Human embryonic stem cells (hESCs), due to their pluripotent nature, represent a particularly relevant model system to study the relationship between the replication program and differentiation state. Here, we define the basic properties of the replication program in hESCs and compare them to the programs of hESC-derived multipotent cells (neural rosette cells) and primary differentiated cells (microvascular endothelial cells [MECs]). We characterized three genomic loci: two pluripotency regulatory genes, POU5F1 (OCT4) and NANOG, and the IGH locus, a locus that is transcriptionally active specifically in B-lineage cells. We applied a high-resolution approach to capture images of individual replicated DNA molecules. We demonstrate that for the loci studied, several basic properties of replication, including the average speed of replication forks and the average density of initiation sites, were conserved among the cells analyzed. We also demonstrate, for the first time, the presence of initiation zones in hESCs. However, significant differences were evident in other aspects of replication for the DNA segment containing the POU5F1 gene. Specifically, the locations of centers of initiation zones and the direction of replication fork progression through the POU5F1 gene were conserved in two independent hESC lines but were different in hESC-derived multipotent cells and MECs. Thus, our data identify features of the replication program characteristic of hESCs and define specific changes in replication during hESC differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.