ArticleVirology journal2010
A systematic approach for the identification of novel, serologically reactive recombinant Varicella-Zoster Virus (VZV) antigens.
Article in Virology journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- An experimental chimeric hepatitis E virus vaccine elicits both local and systemic immune responses.Frontiers in microbiology · 2024Article
- Novel LysM motifs for antigen display on lactobacilli for mucosal immunization.Scientific reports · 2021Article
- Oral Vaccination with Hepatitis E Virus Capsid Protein and Immunobiotic Bacterium-Like Particles Induce Intestinal and Systemic Immunity in Mice.Probiotics and antimicrobial proteins · 2020Article
- A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina.Frontiers in microbiology · 2019Article
- Varicella Virus-Host Interactions During Latency and Reactivation: Lessons From Simian Varicella Virus.Frontiers in microbiology · 2018Review
- Herpes Zoster and Immunogenicity and Safety of Zoster Vaccines in Transplant Patients: A Narrative Review of the Literature.Frontiers in immunology · 2018Review
- The Hepatitis E virus intraviral interactome.Scientific reports · 2015Article
- Systematic reference sample generation for multiplexed serological assays.Scientific reports · 2013Article
- Malaria vaccines: high-throughput tools for antigens discovery with potential for their development.Colombia medica (Cali, Colombia) · 2013Review
- Systematic screening for novel, serologically reactive Hepatitis E Virus epitopes.Virology journal · 2012Article
- RNA interference is responsible for reduction of transgene expression after Sleeping Beauty transposase mediated somatic integration.PloS one · 2012Article
- Varicella zoster virus ORF25 gene product: an essential hub protein linking encapsidation proteins and the nuclear egress complex.Journal of proteome research · 2011Article
- Protein microarrays and biomarkers of infectious disease.International journal of molecular sciences · 2010Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundVaricella-Zoster virus causes chickenpox upon primary infection and shingles after reactivation. Currently available serological tests to detect VZV-specific antibodies are exclusively based on antigens derived from VZV-infected cells.
resultsWe present a systematic approach for the identification of novel, serologically reactive VZV antigens. Therefore, all VZV open reading frames were cloned into a bacterial expression vector and checked for small scale recombinant protein expression. Serum profiling experiments using purified VZV proteins and clinically defined sera in a microarray revealed 5 putative antigens (ORFs 1, 4, 14, 49, and 68). These were rearranged in line format and validated with pre-characterized sera.
conclusionsThe line assay confirmed the seroreactivity of the identified antigens and revealed its suitability for VZV serodiagnostics comparable to commercially available VZV-ELISA. Recombinant ORF68 (gE) proved to be an antigen for high-confidence determination of VZV serostatus. Furthermore, our data suggest that a serological differentiation between chickenpox and herpes zoster may be possible by analysis of the IgM-portfolio against individual viral antigens.
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