ArticleDiabetologia2010
Contribution of postnatally formed small beta cell aggregates to functional beta cell mass in adult rat pancreas.
Article in Diabetologia, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 46 citations in OpenAlex.
- Sustained function of alginate-encapsulated human islet cell implants in the peritoneal cavity of mice leading to a pilot study in a type 1 diabetic patient.Diabetologia · 2013Trial
- Enhanced characterization of beta cell mass in a Tg(BioImpacts : BI · 2022Article
- Functional changes in beta cells during ageing and senescence.Diabetologia · 2020Review
- Inadequate β-cell mass is essential for the pathogenesis of type 2 diabetes.The lancet. Diabetes & endocrinology · 2020Review
- Repurposed Analog of GLP-1 Ameliorates Hyperglycemia in Type 1 Diabetic Mice Through Pancreatic Cell Reprogramming.Frontiers in endocrinology · 2020Article
- RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis.Frontiers in endocrinology · 2020Article
- Cell Mass Increase Associated with Formation of Glucose-Controlling β-Cell Mass in Device-Encapsulated Implants of hiPS-Derived Pancreatic Endoderm.Stem cells translational medicine · 2019Article
- Dexamethasone during pregnancy impairs maternal pancreatic β-cell renewal during lactation.Endocrine connections · 2019Article
- Pancreatic β Cell Regeneration as a Possible Therapy for Diabetes.Cell metabolism · 2018Review
- Stochastic priming and spatial cues orchestrate heterogeneous clonal contribution to mouse pancreas organogenesis.Nature communications · 2017Article
- FUCCI tracking shows cell-cycle-dependent Neurog3 variation in pancreatic progenitors.Genesis (New York, N.Y. : 2000) · 2017Article
- Heterogeneity in the Beta-Cell Population: a Guided Search Into Its Significance in Pancreas and in Implants.Current diabetes reports · 2017Review
- Article
- β-cell replacement sources for type 1 diabetes: a focus on pancreatic ductal cells.Therapeutic advances in endocrinology and metabolism · 2016Review
- STAT3 modulates β-cell cycling in injured mouse pancreas and protects against DNA damage.Cell death & disease · 2016Article
- Dynamic development of the pancreas from birth to adulthood.Upsala journal of medical sciences · 2016Review
- Article
- Determination of Optimal Sample Size for Quantification of β-Cell Area, Amyloid Area and β-Cell Apoptosis in Isolated Islets.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2015Article
- Automated quantification of pancreatic β-cell mass.American journal of physiology. Endocrinology and metabolism · 2014Article
- Glucose regulates rat beta cell number through age-dependent effects on beta cell survival and proliferation.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aims/hypothesisNeogenesis of beta cells and their clustering to small aggregates is a key process in prenatal development of beta cell mass. We investigated the contribution of postnatally formed small aggregates to functional beta cell mass in adult rats.
methodsConditions were defined for (1) counting total beta cell number in pancreases with relative error of <10% and (2) determining their distribution over aggregates of different size and over functionally different subpopulations.
resultsPancreases of 10-week-old male Wistar rats contained 2.8 ± 0.2 × 10⁶ beta cells, of which >90% was generated postnatally, involving: (1) neo-formation of 30,000 aggregates with diameter <50 μm including single cells; and (2) growth of 5,500 aggregates to larger sizes, accounting for 90% of the increase in cell number, with number of growing aggregates in the tail 50% greater than elsewhere. At 10 weeks, 86% of aggregates were <50 μm; compared with aggregates >200 μm, their beta cells exhibited a higher basal insulin content that was also resistant to glibenclamide-induced degranulation. The pool of Ki67-positive beta cells was sixfold larger than at birth and distributed over all aggregate sizes. CONCLUSIONS/
interpretationWe describe a method for in situ counting of beta cell numbers and subpopulations with low relative error. In adult rats, >90% of beta cells and beta cell aggregates are formed after birth. Aggregates <50 μm are more than 100-fold more abundant than aggregates >200 μm, which are selected for isolated islet studies. Their topographic and functional properties contribute to the functional heterogeneity of the beta cell population; their growth to larger aggregates with characteristic beta cell functions may serve future metabolic needs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.