ReviewPlant cell reports2010
Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols.
Review in Plant cell reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 73 citations in OpenAlex.
- Optimization of selection agent concentrations and expanding G418 utility for gentamicin resistance in Marchantia polymorpha.Scientific reports · 2025Article
- Plant genetic transformation: achievements, current status and future prospects.Plant biotechnology journal · 2025Review
- eYGFPuv-Assisted Transgenic Selection inPlants (Basel, Switzerland) · 2023Article
- Multiplexed Transgenic Selection and Counterselection Strategies to Expedite Genetic Manipulation Workflows Using Drosophila melanogaster.Current protocols · 2023Article
- Aminoglycoside Antibiotics Inhibit Phage Infection by Blocking an Early Step of the Infection Cycle.mBio · 2022Article
- Article
- Conjugation of Gentamicin to Polyamidoamine Dendrimers Improved Anti-bacterial Properties againstAdvanced pharmaceutical bulletin · 2021Article
- Efficient Transformation of Somatic Embryos and Regeneration of Cork Oak Plantlets with A Gene (International journal of molecular sciences · 2021Article
- Loss of MAR1 Function is a Marker for Co-Selection of CRISPR-Induced Mutations in Plants.Frontiers in genome editing · 2021Article
- Citrus Genetic Transformation: An Overview of the Current Strategies and Insights on the New Emerging Technologies.Frontiers in plant science · 2021Review
- Genetic Transformation in Peach (Plants (Basel, Switzerland) · 2020Review
- Establishment of an in vitro regeneration system and genetic transformation of the Tunisian 'Maltese half-blood' (3 Biotech · 2020Article
- A robust genetic transformation protocol to obtain transgenic shoots ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Neomycin inhibition of (+)-7-iso-jasmonoyl-L-isoleucine accumulation and signaling.Journal of chemical ecology · 2014Article
- Agrobacterium-mediated genetic transformation of yam (Dioscorea rotundata): an important tool for functional study of genes and crop improvement.Frontiers in plant science · 2014Article
- Generation of Trichoderma atroviride mutants with constitutively activated G protein signaling by using geneticin resistance as selection marker.BMC research notes · 2012Article
- Plant regeneration and stable transformation in the floricultural plant Cleome spinosa, a C₃ plant closely related to the C₄ plant C. gynandra.Plant cell reports · 2012Article
- An antibiotic selection marker for schistosome transgenesis.International journal for parasitology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant transformation protocols generally involve the use of selectable marker genes for the screening of transgenic material. The bacterial gene nptII, coding for a neomycin phosphotransferase, and the hpt gene, coding for a hygromycin phosphotransferase, are frequently used. These enzymes detoxify aminoglycoside antibiotics by phosphorylation, thereby permitting cell growth in the presence of antibiotics. Nevertheless, the screening for transgenic regenerated shoots is often partial and difficult due to regeneration of escapes and chimeras. These difficulties can be caused, in part, by an incorrect assumption about the mode of action of antibiotics in bacterial and eukaryotic cells and in in vitro tissue culture. The information contained in this review could be useful to establish better selection strategies by taking into account factors such as explant complexity, transformation and selection protocols that allow better accessibility to cells of Agrobacterium and antibiotics, and faster regeneration methods that avoid collateral effects of antibiotics on recovered, putative transgenic shoots.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.